Rabbit Polyclonal P38MAPK(Phospho-Thr180) Antibody

The p38 mitogen-activated protein kinase (MAPK) pathway plays a critical role in cellular responses to stress, inflammation, and apoptosis. Phosphorylation at Thr180 (and Tyr182 in the activation loop) is essential for p38 MAPK activation, enabling its kinase activity toward downstream substrates. The Phospho-Thr180 p38 MAPK antibody specifically detects this phosphorylation event, serving as a key tool to monitor p38 activation status in research. This antibody is widely used in techniques like Western blotting, immunofluorescence, and flow cytometry to study signaling dynamics under conditions such as oxidative stress, cytokine stimulation, or drug treatment. Its specificity for the phosphorylated form allows researchers to distinguish active p38 from its inactive state, aiding in the exploration of pathways involving apoptosis, immune responses, and cell differentiation. Applications extend to disease models, including cancer, neurodegenerative disorders, and inflammatory diseases, where dysregulated p38 signaling is implicated. Validation typically includes testing in knockout models or phosphatase-treated samples to confirm target specificity. By tracking Thr180 phosphorylation, this antibody helps unravel the spatiotemporal regulation of p38 activity, contributing to drug discovery and mechanistic studies of cellular adaptation to extracellular signals.