| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK2; MAPKAPK-2; MAPKAPK2 |
| Entrez GeneID | 1432; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 182 (T-G-Y(p)-V-A) derived from Human P38 MAPK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与P38 MAPK(Phospho-Tyr182)抗体相关的参考文献示例(文献信息为模拟概括，实际引用请核实原文)：
---
1. **文献名称**: *"Selective activation of p38 MAPK cascade by Tyr182 phosphorylation in oxidative stress signaling"*
**作者**: Lee JC et al.
**摘要**: 本研究利用Phospho-Tyr182特异性抗体，证实氧化应激通过Tyr182磷酸化选择性激活p38 MAPK通路，并揭示该位点磷酸化在细胞凋亡中的关键作用。
2. **文献名称**: *"p38 MAPK tyrosine phosphorylation modulates its kinase activity in inflammatory response"*
**作者**: Cuenda A, Rousseau S.
**摘要**: 通过Phospho-Tyr182抗体检测，作者发现TNF-α诱导的炎症反应中，Tyr182磷酸化与p38激酶活性直接相关，并调控下游IL-6的产生。
3. **文献名称**: *"Dual phosphorylation of p38 MAPK (Thr180/Tyr182) regulates cancer cell invasion via MMP-9"*
**作者**: Zhang Y et al.
**摘要**: 研究结合Phospho-Tyr182抗体与Phospho-Thr180抗体，证明双位点磷酸化协同促进p38 MAPK介导的肿瘤细胞侵袭，并靶向调控MMP-9表达。
---
注：以上文献为示例性概括，实际研究需通过PubMed/Google Scholar检索关键词“p38 MAPK Tyr182 phosphorylation antibody”获取真实文献。
The p38 mitogen-activated protein kinase (MAPK) pathway is a critical signaling cascade involved in cellular responses to stress, inflammation, apoptosis, and differentiation. Activation of p38 MAPK occurs via dual phosphorylation at conserved Thr180 and Tyr182 residues within its activation loop. Antibodies targeting phosphorylated Tyr182 (Phospho-Tyr182) specifically detect the activated form of p38 MAPK, enabling researchers to study its activation status under various physiological or pathological conditions.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate p38 MAPK signaling in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. They help elucidate mechanisms of drug action, stress responses, and cytokine-mediated signaling. Validation typically includes testing knockout cells or phosphorylation inhibitors to confirm specificity.
When using Phospho-Tyr182 antibodies, proper sample preparation (e.g., phosphatase inhibitors) and controls (e.g., total p38 antibodies) are essential. Cross-reactivity with related phosphorylated MAPKs (e.g., JNK or ERK) should be ruled out. Commercial antibodies often specify host species (e.g., rabbit monoclonal) and recommended applications. Storage at -20°C and avoiding repeated freeze-thaw cycles maintain antibody stability. Always refer to manufacturer datasheets for optimal protocols.
×
