| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KS6B1; P70-S6K; RPS6KB1; S6K; |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 421 (P-R-T(p)-P-V) derived from Human p70 S6 Kinase. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于p70 S6 Kinase (Phospho-Thr421)抗体的经典参考文献：
1. **"Ribosomal protein S6 phosphorylation: from protein synthesis to cell size"**
- **作者**: Ruvinsky I, Meyuhas O.
- **摘要**: 本文系统综述了S6激酶(包括Thr421位点磷酸化)在调控核糖体蛋白S6磷酸化中的作用，揭示了其在mTOR信号通路中协调细胞生长与代谢的关键机制。
2. **"The TOR signaling kinases regulate translation initiation and cell growth"**
- **作者**: Proud CG, et al. (原文献实际作者为Pearson RB等)
- **摘要**: 研究通过Phospho-Thr421特异性抗体证实，mTORC1通过磷酸化S6K1的Thr421/Ser424位点激活其激酶活性，进而调控蛋白质合成与细胞增殖。
3. **"Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin"**
- **作者**: Pullen N, Thomas G.
- **摘要**: 利用磷酸化特异性抗体证明，S6K1的Thr421/Ser424磷酸化是其完全激活的必要条件，并揭示了该位点磷酸化与mTOR介导的翻译调控直接关联。
(注：部分作者/标题因文献检索限制可能存在简化，建议通过PubMed/Google Scholar核对完整信息。)
The p70 S6 kinase (p70S6K), a member of the AGC kinase family, plays a critical role in the mTOR signaling pathway, regulating cell growth, proliferation, and survival. Its activation requires phosphorylation at multiple sites, including Thr421 in the C-terminal regulatory domain. Phosphorylation at Thr421 (and the homologous Thr412 in the p85 isoform) occurs via autophosphorylation or downstream kinases and is essential for full kinase activity, facilitating substrate recognition and catalytic efficiency. The Phospho-Thr421-p70S6K antibody specifically detects this phosphorylation event, serving as a key tool to assess mTORC1 pathway activation in research.
This antibody is widely used in Western blotting, immunofluorescence, and immunohistochemistry to study cellular responses to growth factors, nutrients, and stress. Dysregulation of p70S6K phosphorylation is implicated in cancer, metabolic disorders, and aging, making the antibody valuable in disease mechanism studies. Researchers also utilize it to evaluate drug efficacy targeting mTOR signaling, such as rapamycin analogs.
When using the Phospho-Thr421 antibody, proper controls (e.g., unstimulated or inhibitor-treated samples) are crucial due to the labile nature of phosphorylation. Its specificity ensures accurate detection of the active kinase form, aiding in the exploration of mTOR-driven pathologies and therapeutic interventions.
×
