| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | P105-RB; PP105; PP110; RB1; |
| Entrez GeneID | 5925; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 608(Y-L-S(p)-P-V) derived from Human Retinoblastoma. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Retinoblastoma (Phospho-Ser608)抗体的3篇参考文献(注：由于具体文献的可用性限制，以下内容为模拟示例，建议通过PubMed或学术数据库核实真实文献)：
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1. **文献名称**: *"Phosphorylation of Retinoblastoma Protein at Serine 608 Regulates Cell Cycle Exit in Differentiated Cells"*
**作者**: Smith J, et al.
**摘要**: 研究揭示了Rb蛋白在Ser608位点的磷酸化对细胞周期退出和终末分化的调控作用，通过特异性抗体检测发现该位点的磷酸化水平在G1期显著降低，提示其与E2F活性的抑制相关。
2. **文献名称**: *"A Novel Role of Rb Phosphorylation at Ser608 in DNA Damage Response"*
**作者**: Lee H, et al.
**摘要**: 利用Phospho-Ser608特异性抗体进行免疫印迹分析，发现DNA损伤后该位点的磷酸化增强，表明Rb在Ser608的修饰可能参与调控DNA修复信号通路。
3. **文献名称**: *"Development and Validation of a Phospho-Specific Antibody for Retinoblastoma Protein Serine 608"*
**作者**: Chen L, et al.
**摘要**: 报道了一种针对Rb蛋白Ser608磷酸化位点的多克隆抗体的开发，验证了其在免疫沉淀和免疫荧光中的特异性，并应用于肿瘤样本中Rb活性状态的检测。
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**提示**：若需真实文献，建议在PubMed中以关键词 **"Retinoblastoma Phospho-Ser608 antibody"** 或 **"Rb Ser608 phosphorylation"** 检索，或查阅抗体供应商(如CST、Abcam)的产品引用文献列表。
Retinoblastoma (Phospho-Ser608) antibody is a specialized tool used to detect the phosphorylated form of the retinoblastoma protein (RB1) at serine residue 608. RB1. a tumor suppressor encoded by the RB1 gene, plays a critical role in regulating cell cycle progression by binding to and inhibiting E2F transcription factors, thereby preventing uncontrolled cell division. Phosphorylation of RB1 at specific residues, including Ser608. disrupts its interaction with E2F, enabling cell cycle entry. This phosphorylation event is primarily mediated by cyclin-dependent kinases (CDKs) during the G1-to-S phase transition, serving as a key regulatory mechanism in normal cellular proliferation.
The Retinoblastoma (Phospho-Ser608) antibody is widely utilized in research to study RB1 inactivation dynamics, particularly in cancer biology. Dysregulation of RB1 phosphorylation is associated with tumorigenesis, as hyperphosphorylation leads to loss of tumor suppressor function. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess RB1 phosphorylation status in cell lines, tissue samples, or experimental models. Its specificity for the phosphorylated Ser608 epitope makes it valuable for investigating cell cycle checkpoints, therapeutic responses, and mechanisms of oncogenic signaling pathways. Additionally, it aids in exploring connections between RB1 dysfunction and diseases beyond retinoblastoma, such as osteosarcoma and other cancers with RB1 pathway alterations. Proper validation using phosphorylation-specific controls is essential for experimental accuracy.
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