| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.10.1; High affinity nerve growth factor receptor precursor; kinase TrkA; NTRK1; p140-TrkA; Slow nerve growth factor receptor; TRK; Trk-A; TRK1 transforming tyrosine kinase protein; TRKA |
| Entrez GeneID | 4914; |
| WB Predicted band size | 87kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 701 (I-L-Y(p)-R-K) derived from Human Trk A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Trk A (Phospho-Tyr701)抗体相关的文献摘要示例(注：文献为模拟示例，实际引用需根据真实文献调整)：
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1. **文献名称**：*"NGF-induced TrkA phosphorylation at Tyr701 mediates neuronal differentiation signaling"*
**作者**：Huang EJ, Reichardt LF
**摘要**：研究揭示了NGF通过诱导TrkA受体Tyr701位点的磷酸化，激活下游MAPK/ERK通路，促进PC12细胞神经突生长和分化，该抗体用于Western blot验证磷酸化事件。
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2. **文献名称**：*"Phosphorylation-specific antibody to TrkA Y701 reveals its role in cancer cell invasiveness"*
**作者**：Tacconi C, et al.
**摘要**：利用Phospho-Tyr701抗体在乳腺癌组织样本中检测到TrkA异常激活，证明其与肿瘤转移相关，并通过siRNA实验证实该位点磷酸化调控细胞迁移。
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3. **文献名称**：*"Validation of phospho-specific antibodies for Trk receptor activation states"*
**作者**：Watson FL, et al.
**摘要**：该研究系统验证了多种Trk磷酸化抗体(包括Tyr701)的特异性，证明其在体外激酶实验和小鼠脑组织免疫荧光中的可靠性，为神经疾病模型提供检测工具。
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建议通过PubMed或Google Scholar搜索关键词 **"TrkA Y701 phosphorylation antibody"** 获取真实文献，并优先选择高引用的细胞信号传导或神经生物学领域论文。
The Trk A (Phospho-Tyr701) antibody is designed to detect the activated form of Tropomyosin receptor kinase A (Trk A), a transmembrane receptor tyrosine kinase encoded by the *NTRK1* gene. Trk A primarily binds nerve growth factor (NGF) and plays a critical role in neuronal survival, differentiation, and pain signaling. Upon NGF binding, Trk A undergoes dimerization and autophosphorylation at specific tyrosine residues, including Tyr701. within its intracellular kinase domain. Phosphorylation at Tyr701 is a key activation marker, facilitating downstream signaling through pathways like MAPK/ERK, PI3K/Akt, and PLCγ, which regulate cell growth and apoptosis.
The Trk A (Phospho-Tyr701) antibody specifically recognizes the phosphorylated state of Tyr701. enabling researchers to study Trk A activation status in various contexts, such as neurodevelopment, neurodegenerative diseases, and cancer. Dysregulated Trk A signaling, including hyperphosphorylation, has been implicated in neuroblastoma, breast cancer, and other malignancies. This antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess Trk A activation in cell lines, tissues, or preclinical models. Its application aids in understanding Trk A's role in disease mechanisms and evaluating therapeutic agents targeting NGF/Trk A signaling. Proper controls, including non-phosphorylated Trk A detection and phosphatase-treated samples, are essential to validate specificity.
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