| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1 |
| Entrez GeneID | 4303; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human FoxO4 around the phosphorylation site of S197. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于FoxO4 (Phospho-Ser197)抗体的3篇参考文献示例(内容为模拟概括，非真实文献)：
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1. **文献名称**：*"AKT-mediated phosphorylation of FoxO4 at Ser197 regulates cellular senescence"*
**作者**：Chen L et al.
**摘要**：研究揭示了AKT激酶对FoxO4 Ser197位点的磷酸化调控细胞衰老的机制。通过Phospho-Ser197特异性抗体，作者证实该修饰促进FoxO4的核输出，抑制其转录活性，从而延缓衰老相关分泌表型(SASP)。
2. **文献名称**：*"Development and validation of phospho-specific antibodies targeting FoxO transcription factors"*
**作者**：Tanaka K et al.
**摘要**：本研究描述了一种针对FoxO家族成员(包括FoxO4 Ser197)磷酸化表位的多克隆抗体制备方法。通过肽段免疫和亲和纯化，验证了抗体在Western blot和免疫荧光中的特异性，并应用于氧化应激模型中FoxO4动态磷酸化分析。
3. **文献名称**：*"Phosphorylation of FoxO4 at Ser197 modulates its interaction with 14-3-3 proteins in cancer progression"*
**作者**：Wang Y et al.
**摘要**：利用Phospho-Ser197抗体，研究发现肿瘤微环境中生长因子信号通过该位点磷酸化增强FoxO4与14-3-3蛋白的结合，导致其失活，进而促进肿瘤细胞增殖和转移，为靶向干预提供了新思路。
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**说明**：以上内容为示例性模拟，实际文献需通过PubMed、Web of Science等平台检索关键词如“FoxO4 Ser197 phosphorylation”或“Phospho-Ser197 antibody”获取。若需具体文献，建议补充实验背景(如疾病模型或信号通路)，以便进一步筛选。
The FoxO4 (Phospho-Ser197) antibody is a specialized tool used to detect the phosphorylation status of the Forkhead box O4 (FoxO4) protein at serine residue 197. FoxO4. a member of the Forkhead box O (FoxO) transcription factor family, plays a critical role in regulating cellular processes such as apoptosis, oxidative stress response, cell cycle arrest, and metabolism. Its activity is tightly controlled by post-translational modifications, particularly phosphorylation, which modulates its subcellular localization and transcriptional activity. Phosphorylation at Ser197. often mediated by upstream kinases like Akt (Protein Kinase B) in response to insulin or growth factor signaling, promotes FoxO4's translocation from the nucleus to the cytoplasm, thereby inhibiting its transcriptional functions. This modification is a key regulatory mechanism in pathways linked to cellular survival, aging, and tumor suppression.
The FoxO4 (Phospho-Ser197) antibody is specifically designed to recognize this phosphorylated epitope, enabling researchers to study FoxO4 activation dynamics in various contexts, including cancer, metabolic disorders, and age-related diseases. It is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation-dependent FoxO4 regulation in cell lines or tissue samples. Validated for specificity and sensitivity, this antibody aids in elucidating how dysregulated FoxO4 signaling contributes to pathological conditions, offering insights into therapeutic targeting of the PI3K/Akt/FoxO axis. Its applications span basic research and drug discovery, emphasizing its utility in understanding cellular stress responses and survival mechanisms.
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