| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2 |
| Entrez GeneID | 839; |
| WB Predicted band size | 33kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Caspase-6 around the phosphorylation site of S257. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Caspase-6 (Phospho-Ser257)抗体的3篇示例文献(内容为模拟概括，建议通过学术数据库验证)：
1. **文献名称**：*Phosphorylation of Caspase-6 at Ser257 regulates its enzymatic activity in neurodegenerative models*
**作者**：Smith A, et al.
**摘要**：研究发现Caspase-6在Ser257位点的磷酸化可通过抑制其蛋白酶活性，减少神经元凋亡，为阿尔茨海默病治疗提供新靶点。
2. **文献名称**：*A novel kinase-mediated mechanism of Caspase-6 activation in apoptosis*
**作者**：Zhang L, et al.
**摘要**：揭示了激酶PKCα对Caspase-6 Ser257位点的磷酸化调控机制，证明该修饰促进Caspase-6在癌细胞凋亡中的激活及底物切割能力。
3. **文献名称**：*Site-specific phosphorylation of Caspase-6 modulates its role in viral infection response*
**作者**：Kim J, et al.
**摘要**：通过磷酸化抗体检测发现，病毒感染诱导的Ser257磷酸化抑制Caspase-6介导的宿主细胞死亡，提示其参与免疫逃逸通路。
**注意**：以上为基于研究领域常见方向的模拟示例，实际文献需通过PubMed或Google Scholar以关键词“Caspase-6 Ser257 phosphorylation”检索确认。
Caspase-6. a member of the cysteine-aspartic protease family, plays critical roles in apoptosis, inflammation, and cellular differentiation. It is synthesized as an inactive procaspase (zymogen) and undergoes proteolytic cleavage to form active subunits (p18 and p11). Caspase-6 is involved in cleaving substrates like lamin A/C and amyloid precursor protein (APP), linking it to apoptotic nuclear disintegration and neurodegenerative pathologies. Phosphorylation at Ser257. located near the catalytic site, is a key post-translational modification regulating its activity. Studies suggest this phosphorylation may modulate enzyme activation, substrate binding, or interaction with regulatory proteins, though precise mechanisms remain under investigation.
The Caspase-6 (Phospho-Ser257) antibody specifically detects caspase-6 phosphorylated at serine 257. enabling researchers to study its activation status and regulatory pathways. This antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore caspase-6's role in physiological and pathological contexts, including Alzheimer’s disease, Huntington’s disease, and cancer. Dysregulated caspase-6 activity, particularly its phosphorylated form, has been implicated in neuronal death and disease progression, making this antibody a valuable tool for understanding disease mechanisms and therapeutic targeting. Its specificity for the phosphorylated epitope ensures accurate detection in complex biological samples.
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