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Rabbit Polyclonal PEPC(Phospho-Ser15) Antibody

  • 中文名: PEPC (Phospho-Ser15)抗体
  • 别    名: PGC; Gastricsin; Pepsinogen C
货号: IPDX40773
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Plant
IF 咨询技术 Plant
IHC 咨询技术 Plant
ICC 技术咨询 Plant
FCM 咨询技术 Plant
Elisa 咨询技术 Plant

产品详情

AliasesPGC; Gastricsin; Pepsinogen C
Entrez GeneID5225;
WB Predicted band size105-110kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityPlant
ImmunogenPhospho peptide at Ser15 of PEPC
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是3-4篇假设性参考文献示例(仅供参考,具体文献需通过学术数据库检索确认):

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1. **文献名称**:*Light-dependent phosphorylation of PEP carboxylase in Arabidopsis thaliana*

**作者**:Smith J, Doe R, Chen L

**摘要**:本研究利用Phospho-Ser15特异性抗体,通过Western blot分析拟南芥叶片中PEPC的磷酸化动态。结果表明,光照通过激活激酶调控Ser15位点磷酸化,进而增强PEPC活性以促进光合碳固定。

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2. **文献名称**:*Regulation of PEPC activity by reversible phosphorylation in C4 plants*

**作者**:Zhang Y, Wang H, Nakamura K

**摘要**:通过Phospho-Ser15抗体的免疫印迹和免疫沉淀技术,作者揭示了C4植物玉米中PEPC磷酸化与昼夜节律的关系,发现Ser15磷酸化负反馈调控酶活性以适应光暗周期变化。

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3. **文献名称**:*Drought stress induces dynamic phosphorylation of PEP carboxylase in sorghum*

**作者**:Tanaka M, Rodriguez-Sanchez R

**摘要**:该研究使用Phospho-Ser15抗体检测高粱PEPC在干旱胁迫下的磷酸化修饰,发现Ser15位点磷酸化水平显著升高,可能通过改变代谢通量增强植物抗旱性。

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4. **文献名称**:*Structural insights into PEPC phosphorylation at Ser15: A conserved regulatory mechanism*

**作者**:Lee S, Kim T, Ohta D

**摘要**:结合X射线晶体学和Phospho-Ser15抗体验证,本文阐明了Ser15磷酸化引起PEPC构象变化的分子机制,提示该位点在多种植物中的保守调控功能。

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**注**:以上为假设案例,实际文献需通过关键词“PEPC phosphorylation Ser15 antibody”在PubMed、Web of Science等平台检索,推荐筛选实验方法中明确使用该抗体的研究。

背景信息

The PEPC (Phospho-Ser15) antibody is designed to detect the phosphorylated form of phosphoenolpyruvate carboxylase (PEPC) at serine residue 15. a key post-translational modification regulating enzyme activity. PEPC is a critical metabolic enzyme in plants, bacteria, and some eukaryotes, catalyzing the irreversible carboxylation of phosphoenolpyruvate (PEP) to oxaloacetate. This reaction is central to carbon fixation in C4 and CAM plants, nitrogen assimilation, and amino acid synthesis. Phosphorylation at Ser15 in plant PEPC (e.g., in maize or Arabidopsis) modulates enzyme kinetics by reducing sensitivity to allosteric inhibitors like malate, thereby enhancing catalytic activity during light-induced activation. This regulatory mechanism allows plants to optimize carbon flux under varying environmental conditions, such as light-dark cycles or stress. The Phospho-Ser15 antibody is widely used in plant physiology research to study PEPC regulation, metabolic adaptations, and responses to abiotic stresses. It enables detection of phosphorylated PEPC via techniques like Western blotting or immunoprecipitation, providing insights into enzyme activation states. Cross-reactivity with PEPC homologs in other species should be validated experimentally. This tool has advanced understanding of carbon metabolism dynamics and potential biotechnological applications for improving crop resilience.

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