| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/20-1/100 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ESP1; SEPA |
| Entrez GeneID | 9700; |
| WB Predicted band size | 233kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S1126 of human ESPL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于ESPL1(Phospho-S1126/801)抗体的参考文献示例(内容为模拟，建议通过学术数据库验证真实性)：
---
1. **文献名称**: *Phosphorylation of ESPL1 at Serine 1126 regulates sister chromatid separation during mitosis*
**作者**: Li, X., et al.
**摘要**: 本研究揭示了ESPL1在S1126位点的磷酸化对其蛋白酶活性的调控作用，通过特异性抗体证实该修饰在有丝分裂中期到后期的转换中至关重要，影响姐妹染色体的及时分离。
2. **文献名称**: *DNA damage-induced phosphorylation of ESPL1 at Ser801 promotes genomic stability*
**作者**: Wang, Y., et al.
**摘要**: 利用Phospho-S801抗体，作者发现DNA损伤信号通过ATM激酶磷酸化ESPL1.增强其与染色体结合的能力，从而防止异常染色体分离，维持基因组稳定性。
3. **文献名称**: *Dynamics of ESPL1 phosphorylation in cell cycle progression*
**作者**: Tanaka, K., & Patel, S.
**摘要**: 通过免疫印迹和免疫荧光分析，该研究系统比较了S1126/S801双位点磷酸化的时相变化，提出两者协同调控ESPL1的亚细胞定位及底物切割效率。
4. **文献名称**: *Development and validation of a phospho-specific antibody for ESPL1 in cancer models*
**作者**: Chen, R., et al.
**摘要**: 报道了一种高特异性Phospho-S1126/801抗体的制备与验证，并应用于乳腺癌模型，证明ESPL1异常磷酸化与化疗耐药性相关。
---
建议通过**PubMed**或**Google Scholar**以关键词“ESPL1 phosphorylation S1126/S801 antibody”检索最新文献，或查阅抗体供应商(如CST、Abcam)提供的引用文献列表。
The ESPL1 (Phospho-S1126/801) antibody is a specialized tool used to study the phosphorylation status of separase, a cysteine protease encoded by the *ESPL1* gene. Separase plays a critical role in cell cycle regulation by cleaving cohesin complexes during anaphase, enabling sister chromatid separation. Its activity is tightly controlled via phosphorylation at specific residues, including Serine 1126 (S1126) and Serine 801 (S801), which are associated with regulatory checkpoints. Phosphorylation at these sites modulates separase’s enzymatic activity, ensuring timely chromosome segregation and genomic stability. Dysregulation of separase has been linked to chromosomal instability disorders and cancers, highlighting its biological significance.
This antibody is designed to detect endogenous levels of ESPL1 only when phosphorylated at S1126/S801. making it valuable for investigating cell cycle dynamics, DNA damage responses, or mitotic errors. It is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess separase activation states in research models. Studies utilizing this antibody may explore how phosphorylation impacts separase’s interaction with inhibitors (e.g., securin) or its role in pathological conditions. Validation typically includes testing in knockout or phosphatase-treated samples to confirm specificity. Researchers in cancer biology or cell cycle regulation often employ this reagent to dissect mechanisms underlying genome integrity and therapeutic targets.
×
