Rabbit Polyclonal Dnmt1(Phospho-Ser714) Antibody

The Dnmt1 (Phospho-Ser714) antibody is a specialized tool used to detect the phosphorylation of DNA methyltransferase 1 (Dnmt1) at serine residue 714. Dnmt1 is a key enzyme responsible for maintaining DNA methylation patterns during replication, ensuring epigenetic stability by copying methylation marks from parental to daughter DNA strands. Post-translational modifications, such as phosphorylation, regulate Dnmt1's activity, localization, and interactions with other proteins. Phosphorylation at Ser714 has been implicated in modulating Dnmt1 function during the cell cycle, particularly in S phase, where its activity is critical for preserving methylation fidelity. Studies suggest that this modification may influence Dnmt1's association with replication machinery or its recruitment to chromatin. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to investigate regulatory mechanisms of DNA methylation in processes like cellular differentiation, cancer (where Dnmt1 dysregulation is common), or aging. Validating the antibody's specificity via knockout/knockdown controls or peptide competition is essential, as cross-reactivity with other phospho-epitopes could lead to misinterpretation. Understanding Ser714 phosphorylation dynamics provides insights into epigenetic regulation and potential therapeutic targeting in diseases linked to DNA methylation defects.