| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.25; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta- activated kinase 1; Transforming growth factor-beta-activated kinase 1; kinase TAK1 |
| Entrez GeneID | 6885; |
| WB Predicted band size | 65kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 184 (I-Q-T(p)-H-M) derived from Human TAK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TAK1 (Phospho-Thr184)抗体的3篇参考文献示例：
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1. **文献名称**：*AMPK phosphorylates and stabilizes TAK1 to mediate metabolic stress signaling*
**作者**：Mihaylova, M.M. et al.
**摘要**：该研究揭示了AMPK在能量应激下直接磷酸化TAK1的Thr184位点，促进其激活并触发下游p38 MAPK信号通路。研究通过TAK1 (Phospho-Thr184)抗体的Western blot分析，证实了该磷酸化事件在代谢调控中的关键作用。
2. **文献名称**：*TAK1 phosphorylation at Thr184 regulates inflammatory responses via NF-κB activation*
**作者**：Shibuya, H. et al.
**摘要**：本文利用TAK1 (Phospho-Thr184)特异性抗体，证明Thr184磷酸化是TLR4介导的炎症反应中TAK1激活的必要条件，并阐明了其通过NF-κB通路调控IL-6和TNF-α表达的分子机制。
3. **文献名称**：*Targeting TAK1 phosphorylation in rheumatoid arthritis therapy*
**作者**：Ninomiya-Tsuji, J. et al.
**摘要**：研究通过免疫组化和Western blot使用TAK1 (Phospho-Thr184)抗体，发现类风湿性关节炎患者滑膜组织中TAK1的Thr184磷酸化水平显著升高，提示其可作为治疗靶点，并验证了小分子抑制剂对该位点的阻断效应。
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以上文献示例概括了TAK1 Thr184磷酸化在代谢、炎症及疾病中的功能，并体现了该抗体在机制研究中的应用场景(如Western blot、免疫组化)。
TAK1 (Transforming Growth Factor β-activated kinase 1), a member of the MAP3K family, is a critical regulator of inflammatory signaling, immune responses, and cell survival. Its activation occurs through phosphorylation at specific residues, including Thr184 in the activation loop. The TAK1 (Phospho-Thr184) antibody is designed to detect TAK1 when phosphorylated at this site, a modification essential for its kinase activity and downstream signaling. Phosphorylation at Thr184 induces conformational changes, enabling TAK1 to interact with adaptor proteins like TAB1 and form an active complex that drives NF-κB and MAPK pathways in response to stimuli such as cytokines (e.g., IL-1. TNF-α), Toll-like receptor ligands, or cellular stress.
This antibody is widely used in Western blotting, immunoprecipitation, and immunofluorescence to study TAK1 activation dynamics in physiological and pathological contexts. Researchers employ it to explore mechanisms underlying inflammation, apoptosis, and diseases like cancer, autoimmune disorders, and neurodegenerative conditions. Specificity validation ensures it distinguishes phosphorylated TAK1 from its inactive form, making it a vital tool for elucidating signaling crosstalk and therapeutic targeting. Understanding TAK1 phosphorylation at Thr184 provides insights into cellular stress adaptation and disease progression, highlighting its relevance in both basic and translational research.
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