Rabbit Polyclonal STAT5B(Phospho-Ser731) Antibody

The STAT5B (Phospho-Ser731) antibody is designed to detect STAT5B protein when phosphorylated at serine residue 731. a post-translational modification critical for regulating its activity. STAT5B, a member of the Signal Transducer and Activator of Transcription (STAT) family, plays a key role in transmitting signals from cytokines, growth factors, and hormones to the nucleus, driving gene expression involved in cell proliferation, differentiation, and survival. While STAT5 activation classically involves tyrosine phosphorylation (e.g., Tyr699) to facilitate dimerization and nuclear translocation, phosphorylation at Ser731 represents a regulatory mechanism that fine-tunes its transcriptional activity. Studies suggest that Ser731 phosphorylation may modulate STAT5B interactions with co-regulators or influence its DNA-binding affinity, potentially acting as a feedback control to limit sustained signaling. Dysregulation of STAT5B phosphorylation is linked to cancers, immune disorders, and metabolic diseases. The STAT5B (Phospho-Ser731) antibody is widely used in research to investigate context-specific phosphorylation dynamics in cell lines, tissues, or disease models via techniques like Western blotting, immunoprecipitation, or immunofluorescence. Its specificity for the phosphorylated form enables precise analysis of STAT5B activation states, aiding in studies of signaling pathways, drug responses, and pathological mechanisms. Validation typically includes knockout controls or peptide competition assays to ensure minimal cross-reactivity with non-phosphorylated STAT5B or other STAT isoforms.