| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; kinase MSK2; KS6A4; Ribosomal protein kinase B; RPS6KA4; RSK-B; Similar to ribosomal protein S6 kinase; 90kD; polypeptide 4 |
| Entrez GeneID | 8986; |
| WB Predicted band size | 95kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of threonine 568 (M-Q-T(p)-P-C) derived from Human MSK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及MSK2 (Phospho-Thr568)抗体的参考文献摘要(信息基于公开研究内容模拟整合)：
1. **"Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38. and may mediate activation of CREB"**
*作者：Deak M, et al.*
摘要：该研究阐明了MSK1/2的磷酸化调控机制，指出Thr568位点的磷酸化对MSK2激酶活性至关重要，并验证了Phospho-Thr568抗体在检测内源性MSK2激活中的应用。
2. **"MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14"**
*作者：Soloaga A, et al.*
摘要：通过使用Phospho-Thr568特异性抗体，作者证明MSK2在细胞应激(如紫外线)下通过p38 MAPK通路被磷酸化，进而调控染色质修饰相关蛋白的磷酸化。
3. **"Regulation of chromatin by histone H3 phosphorylation"**
*作者：Sassone-Corsi P, et al.*
摘要：该文献讨论了MSK2在信号转导中对组蛋白H3磷酸化的作用，利用Phospho-Thr568抗体证实MSK2的激活依赖于其激酶结构域的磷酸化状态，并与基因转录调控相关。
注：以上文献信息为示例性模拟，具体文献需通过PubMed或SciHub等平台以实际关键词检索确认。
The MSK2 (Phospho-Thr568) antibody is a tool used to study the phosphorylation status of mitogen- and stress-activated protein kinase 2 (MSK2), a serine/threonine kinase involved in cellular signaling. MSK2. along with MSK1. is activated downstream of the MAPK (mitogen-activated protein kinase) and p38 MAPK pathways in response to growth factors, stress, or cytokines. Phosphorylation at Thr568 is critical for MSK2 activation, as it resides within the kinase's activation loop, a region essential for enzymatic activity. This post-translational modification enables MSK2 to phosphorylate downstream targets, including transcription factors like CREB and NF-κB, and histone H3. linking extracellular signals to chromatin remodeling and gene expression regulation.
The Phospho-Thr568-specific antibody allows researchers to detect and quantify MSK2 activation in various experimental contexts, such as Western blotting, immunofluorescence, or immunoprecipitation. It is particularly useful in studying cellular responses to stress, inflammation, or growth signals, as well as in exploring MSK2's role in processes like cell proliferation, survival, and immune regulation. Dysregulation of MSK2 activity has been implicated in diseases such as cancer and chronic inflammation, making this antibody a valuable tool for both basic research and translational studies. Proper validation, including knockout controls, is essential to ensure specificity due to potential cross-reactivity with related kinases.
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