| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein; FRP1; kinase ATR; protein kinase ATR |
| Entrez GeneID | 545; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 428 (G-I-S(p)-P-K) derived from Human ATR. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于ATR (Phospho-Ser428)抗体的模拟参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*"Phosphorylation of ATR at Serine 428 regulates its activation in response to DNA damage"*
**作者**：Smith J, et al.
**摘要**：研究通过开发特异性识别ATR Ser428磷酸化位点的抗体，验证了该位点在DNA损伤后ATR激活中的关键作用，并证明其在细胞周期检查点调控中的应用。
2. **文献名称**：*"A monoclonal antibody targeting phospho-Ser428-ATR reveals its role in replication stress signaling"*
**作者**：Lee H, et al.
**摘要**：报道了一种高特异性单克隆抗体的开发，该抗体能选择性识别ATR的Ser428磷酸化形式，应用于免疫印迹和免疫荧光，揭示了ATR在复制应激条件下对CHK1通路的调控机制。
3. **文献名称**：*"Validation of a phospho-specific ATR antibody for clinical cancer research"*
**作者**：Chen R, et al.
**摘要**：通过质谱和抑制实验验证了ATR (Phospho-Ser428)抗体的特异性，并证明其在肿瘤组织样本中检测ATR激活状态的应用潜力，为癌症治疗靶向研究提供工具。
4. **文献名称**：*"Dynamic phosphorylation of ATR at Ser428 during mitosis and its functional implications"*
**作者**：Garcia M, et al.
**摘要**：利用Phospho-Ser428抗体发现ATR在细胞有丝分裂期的磷酸化动态变化，提示其可能参与染色体稳定性的非经典调控路径。
(注：以上文献为示例，实际文献需通过PubMed或Google Scholar等平台检索关键词“ATR Ser428 phosphorylation antibody”获取。)
The ATR (Ataxia Telangiectasia and Rad3-related) kinase is a critical regulator of the DNA damage response (DDR), primarily activated during replication stress or DNA damage to stabilize stalled replication forks and coordinate repair. Phosphorylation at Serine 428 (Ser428) is associated with ATR activation, a process triggered by its recruitment to single-stranded DNA (ssDNA) coated by replication protein A (RPA). This phosphorylation event is thought to reflect ATR’s kinase activity and its role in signaling downstream targets, such as CHK1. to arrest the cell cycle and promote DNA repair or apoptosis.
The ATR (Phospho-Ser428) antibody specifically detects ATR when phosphorylated at Ser428. serving as a valuable tool for studying ATR activation dynamics in response to genotoxic stressors like UV radiation, chemotherapeutic agents, or replication inhibitors. Researchers utilize this antibody in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to assess ATR activation status in various experimental models, including cancer cells or tissues.
This antibody is particularly useful in cancer research, as ATR inhibition is being explored to sensitize tumors with defective DDR pathways (e.g., BRCA mutations) to chemotherapy. It also aids in elucidating mechanisms of drug resistance and identifying biomarkers for therapeutic response. Proper validation, including knockout controls and stimulation with DNA-damaging agents, ensures specificity in detecting the phosphorylated form, minimizing cross-reactivity with related kinases like ATM. Overall, the ATR (Phospho-Ser428) antibody is essential for dissecting DDR pathways and advancing targeted cancer therapies.
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