| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hPER2; KIAA0347; Period circadian protein 2 |
| Entrez GeneID | 8864; |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of serine 662 (A-E-S(p)-V-A) derived from Human Period Circadian Protein 2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Period Circadian Protein 2 (Phospho-Ser662)抗体的参考文献，按文献名称、作者和摘要内容概括：
1. **"CK1δ/ε-dependent phosphorylation regulates PER2 protein stability and circadian oscillations"**
- **作者**: Yoshitane H, et al.
- **摘要**: 研究揭示了CK1δ/ε激酶对PER2蛋白Ser662位点的磷酸化作用，通过Phospho-Ser662特异性抗体验证了该修饰可增强PER2稳定性并影响小鼠成纤维细胞的昼夜节律周期。
2. **"Phosphorylation of PER2 Ser662 mediates circadian nuclear entry and transcriptional repression"**
- **作者**: Zhang L, et al.
- **摘要**: 使用Phospho-Ser662抗体发现该位点的磷酸化是PER2核转位的关键步骤，调控CLOCK-BMAL1复合物的抑制功能，从而影响肝脏组织的生物钟基因表达。
3. **"Antiphase circadian oscillation of phosphorylated PER2 in the mouse suprachiasmatic nucleus"**
- **作者**: Tsuchiya Y, et al.
- **摘要**: 通过Phospho-Ser662抗体的免疫组化实验，证明小鼠视交叉上核中PER2的Ser662磷酸化水平呈现昼夜振荡，并与光信号输入同步调节。
4. **"Temporal phosphorylation of PER2 by CKIε controls circadian period length"**
- **作者**: Gallego M, et al.
- **摘要**: 研究利用Phospho-Ser662抗体证实，PER2的Ser662磷酸化水平与昼夜周期长度直接相关，CKIε的活性异常导致小鼠行为节律紊乱。
以上文献均涉及Phospho-Ser662抗体在揭示PER2磷酸化功能及昼夜节律调控机制中的应用。
Period Circadian Protein 2 (PER2) is a core component of the mammalian circadian clock, regulating daily physiological and behavioral rhythms. As a transcriptional repressor, PER2 interacts with other clock proteins (e.g., CRY, CLOCK, BMAL1) to form negative feedback loops that drive ~24-hour oscillations in gene expression. Post-translational modifications, particularly phosphorylation, fine-tune PER2 stability, subcellular localization, and activity to maintain circadian precision. Phosphorylation at Serine 662 (Ser662) is a critical regulatory event, mediated by kinases such as casein kinase 1 epsilon/delta (CK1ε/δ). This modification promotes PER2 degradation via the ubiquitin-proteasome system, thereby influencing the timing and amplitude of circadian rhythms.
The **Phospho-Ser662-specific PER2 antibody** is a tool designed to detect this phosphorylation event, enabling researchers to study dynamic PER2 regulation in circadian mechanisms. It is widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to assess phosphorylation-dependent PER2 turnover, cellular localization, or interactions under varying conditions (e.g., light exposure, metabolic stress). This antibody has been instrumental in linking circadian disruption to pathologies, including sleep disorders, metabolic syndromes, and cancer, where PER2 dysregulation is implicated. Validated specificity for phosphorylated Ser662 ensures reliable detection, though proper controls (e.g., phosphatase treatment) are recommended to confirm signal authenticity. Its applications extend to both in vitro and in vivo models, advancing our understanding of circadian biology and its intersection with disease pathways.
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