| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | cyclin D2; G1/S-specific cyclin D2 |
| Entrez GeneID | 894; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 280 (A-S-T(p)-P-T) derived from Human Cyclin D2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Cyclin D2 (Phospho-Thr280)抗体的3篇参考文献示例(内容基于学术文献的常见研究方向概括，非真实文献)：
1. **文献名称**：*"Phosphorylation of Cyclin D2 at Threonine 280 regulates its subcellular localization and stability in breast cancer cells"*
**作者**：Smith J, et al.
**摘要**：研究通过Western blot和免疫荧光技术，利用Cyclin D2 (Phospho-Thr280)特异性抗体，揭示了Thr280位点磷酸化对Cyclin D2核质穿梭及蛋白稳定性的调控机制，并发现其在乳腺癌中异常高表达与不良预后相关。
2. **文献名称**：*"A novel role of Cyclin D2 phosphorylation in leukemia cell proliferation: Insights from phospho-specific antibody-based assays"*
**作者**：Chen L, et al.
**摘要**：该研究使用Phospho-Thr280 Cyclin D2抗体，通过流式细胞术和免疫沉淀技术，证明Thr280磷酸化在急性髓系白血病细胞中促进Cyclin D2与CDK4/6复合物的结合，从而驱动细胞周期进展。
3. **文献名称**：*"Cyclin D2 phosphorylation at Thr280 mediates neuronal apoptosis in Alzheimer’s disease models"*
**作者**：Wang Y, et al.
**摘要**：利用该抗体进行脑组织切片染色和体外神经元实验，发现Thr280磷酸化Cyclin D2在阿尔茨海默病模型中异常激活，导致神经元细胞周期异常再进入和凋亡，为神经退行性疾病的机制提供了新视角。
4. **文献名称**：*"Development and validation of a phospho-specific Cyclin D2 (Thr280) antibody for cancer biomarker studies"*
**作者**：Johnson R, et al.
**摘要**：文章详细描述了该抗体的制备流程(如抗原设计、单克隆筛选)，并通过ELISA、免疫组化等方法验证其特异性，证明其在结直肠癌组织中可作为潜在诊断标志物。
(注：以上文献为示例性概括，实际引用需查询真实数据库如PubMed、Web of Science。)
Cyclin D2 (Phospho-Thr280) antibodies are specialized tools used to detect the phosphorylation of cyclin D2 at threonine residue 280. a post-translational modification critical for regulating its function in cell cycle progression. Cyclin D2. a member of the D-type cyclin family, plays a key role in driving the G1-to-S phase transition by binding and activating cyclin-dependent kinases (CDKs) 4/6. Phosphorylation at Thr280 is associated with cyclin D2 stability and subcellular localization. This modification, mediated by kinases such as glycogen synthase kinase-3β (GSK-3β), marks cyclin D2 for nuclear export and subsequent proteasomal degradation, thereby influencing cell cycle exit or progression.
Dysregulation of cyclin D2 phosphorylation is implicated in various diseases, particularly cancers. Overexpression or aberrant phosphorylation of cyclin D2 can disrupt normal cell cycle control, promoting uncontrolled proliferation. Antibodies targeting phospho-Thr280 enable researchers to study these mechanisms in models of tumorigenesis, drug response, or metabolic signaling. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation status in cell lines, tissues, or preclinical samples. Additionally, these antibodies aid in exploring links between cyclin D2 dynamics and therapeutic resistance, offering insights for targeted cancer therapies. Validation using phosphorylation-specific controls (e.g., kinase inhibitors or phospho-mutants) is essential to ensure antibody specificity.
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