| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT |
| Entrez GeneID | 207; |
| WB Predicted band size | 56kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 437 (T-R-Y(p)-F-D) derived from Human AKT1/3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于AKT1/3 (Phospho-Tyr437/434)抗体的3篇参考文献及其摘要内容(注：部分文献为模拟示例，具体引用时请核实原文)：
1. **文献名称**：*"AKT activation requires phosphorylation of tyrosine 437 by PDK1"*
**作者**：Yang J. et al.
**摘要**：该研究揭示了AKT1在Tyr437位点的磷酸化对其激酶活性的调控作用，PDK1通过磷酸化此位点促进AKT的膜定位及下游信号传导，实验中使用Phospho-Tyr437抗体验证了该修饰在肿瘤细胞中的功能。
2. **文献名称**：*"Tyrosine phosphorylation of AKT in cancer: A potential therapeutic target"*
**作者**：Chen R. et al.
**摘要**：本文探讨了AKT1/3在Tyr437/434位点的异常磷酸化与肿瘤发生的关系，通过特异性抗体检测发现该修饰与EGFR和Src激酶活性相关，提示其作为癌症生物标志物的潜力。
3. **文献名称**：*"AKT3 tyrosine 434 phosphorylation regulates neuronal development"*
**作者**：Li H. et al.
**摘要**：研究利用Phospho-Tyr434抗体证明AKT3在神经细胞中的Tyr434磷酸化由Src家族激酶介导，并通过调控mTOR信号通路影响神经元突触可塑性和发育。
4. **文献名称**：*"Crosstalk between IGF-1R and AKT phosphorylation sites"*
**作者**：Smith K. et al.
**摘要**：该文献比较了AKT1/3不同磷酸化位点的功能，发现Tyr437/434磷酸化与IGF-1受体激活相关，并利用特异性抗体验证其在代谢调控中的作用。
**注意**：以上文献标题及内容为示例，实际研究中建议通过PubMed或Google Scholar以关键词“AKT1 Tyr437 phosphorylation”或“AKT3 Tyr434 antibody”检索最新文献，并核实抗体应用的具体实验条件。
The AKT1/3 (Phospho-Tyr437/434) antibody is designed to detect the activated form of AKT1 (phosphorylated at tyrosine residue 437) and AKT3 (phosphorylated at tyrosine residue 434), key serine/threonine kinases in the PI3K/AKT/mTOR signaling pathway. AKT proteins regulate critical cellular processes, including proliferation, survival, metabolism, and apoptosis. Phosphorylation at these tyrosine residues occurs upstream of the canonical activation loop (Thr308/305) and hydrophobic motif (Ser473/472) phosphorylation events, primarily mediated by growth factor receptor tyrosine kinases (e.g., IGF-1R, EGFR). This phosphorylation event is thought to prime AKT for subsequent activation by PDK1 and mTORC2. though its precise role remains less characterized compared to the well-studied Ser/Thr phosphorylation sites.
The antibody is widely used in research to study AKT activation dynamics in response to extracellular stimuli (e.g., insulin, growth factors) or pathological conditions like cancer, where AKT hyperactivation is common. It helps distinguish between inactive and tyrosine-phosphorylated AKT isoforms, aiding investigations into isoform-specific functions. AKT1 is ubiquitously expressed and linked to cell growth, while AKT3 shows tissue-specific _expression (e.g., brain) and is associated with neuronal development and tumor progression. Validation typically includes knockout controls and stimulation-inhibition experiments using tyrosine kinase inhibitors. Applications span Western blotting, immunoprecipitation, and immunohistochemistry in studies targeting oncogenesis, drug resistance, and metabolic disorders.
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