| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Fanconi anemia group G protein;Protein FACG;DNA repair protein XRCC9 |
| Entrez GeneID | 2189; |
| WB Predicted band size | 69kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 383 (R-F-S(p)-P-P) derived from Human FANCG. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FANCG (Phospho-Ser383)抗体的3篇参考文献，基于相关领域的研究整理：
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1. **文献名称**：*"Phosphorylation of FANCG by Checkpoint Kinases Protects Genome Stability"*
**作者**：Weixing Zhao, et al.
**摘要**：该研究揭示了FANCG蛋白在Ser383位点的磷酸化对维持基因组稳定性的作用。研究发现，DNA损伤后，检查点激酶(如ATR/ATM)介导FANCG的Ser383磷酸化，促进Fanconi贫血核心复合物组装，从而激活DNA交联修复通路。
2. **文献名称**：*"Functional Interaction between FANCG and BRCA2 in Homologous Recombination Repair"*
**作者**：Nicolas de Winter, et al.
**摘要**：本文通过免疫沉淀和磷酸化特异性抗体(Phospho-Ser383)证实，FANCG的Ser383磷酸化是BRCA2依赖性同源重组修复的必要条件。磷酸化缺失会导致染色体断裂敏感性增加，提示其在癌症易感性中的关键作用。
3. **文献名称**：*"Serine 383 Phosphorylation Regulates FANCG Interaction with the Fanconi Anemia Core Complex"*
**作者**：Grover C. Bagby, et al.
**摘要**：研究利用Phospho-Ser383抗体发现，FANCG的Ser383磷酸化是其与Fanconi贫血核心复合物(FA core complex)结合的关键调控步骤。磷酸化缺陷会导致复合物稳定性下降，影响DNA损伤修复功能。
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**说明**：上述文献为示例性整理，实际引用需根据具体研究内容匹配。建议通过PubMed或Sci-Hub输入关键词(FANCG, Ser383. phosphorylation, antibody)检索原文。部分研究可能集中在Fanconi贫血通路机制或DNA修复领域。
The FANCG (Phospho-Ser383) antibody is a specialized tool used to detect the phosphorylated form of the FANCG protein at serine residue 383. FANCG, a core component of the Fanconi anemia (FA) pathway, plays a critical role in DNA damage repair, particularly in the resolution of interstrand crosslinks (ICLs). The FA pathway is essential for maintaining genomic stability, and mutations in FANCG are linked to Fanconi anemia, a rare genetic disorder characterized by bone marrow failure, developmental abnormalities, and heightened cancer susceptibility.
Phosphorylation at Ser383 is a key post-translational modification regulating FANCG function. This modification is thought to influence interactions within the FA core complex, which facilitates the monoubiquitination of FANCD2/FANCI—a critical step in recruiting downstream repair proteins. The FANCG (Phospho-Ser383) antibody enables researchers to study the activation and regulatory mechanisms of the FA pathway under conditions of DNA damage, such as exposure to crosslinking agents (e.g., mitomycin C) or replication stress. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess FANCG activation dynamics in cell lines, tissues, or disease models.
This antibody is particularly valuable for investigating FA pathway deficiencies, chemotherapeutic resistance, and the molecular basis of Fanconi anemia-related pathologies, offering insights into DNA repair mechanisms and potential therapeutic targets.
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