Rabbit Polyclonal ATRIP(Phospho-Ser224) Antibody

The ATRIP (Phospho-Ser224) antibody is a specialized tool used to study the activation and regulatory mechanisms of the ATRIP protein, a critical component of the ATR (ataxia-telangiectasia and Rad3-related)-mediated DNA damage response (DDR) pathway. ATRIP interacts with ATR kinase to form a complex essential for stabilizing stalled replication forks and signaling replication stress. Phosphorylation at Ser224 on ATRIP is a key post-translational modification triggered during DNA damage or replication stress, facilitating the recruitment and activation of the ATR-ATRIP complex to single-stranded DNA (ssDNA) coated by replication protein A (RPA). This phosphorylation event is crucial for amplifying DDR signaling, which includes cell cycle checkpoint activation (e.g., via CHK1) and repair processes. The antibody specifically detects ATRIP phosphorylated at Ser224. enabling researchers to investigate ATRIP’s activation status in contexts like genomic instability, cancer biology, or responses to genotoxic therapies. It is commonly validated for techniques such as Western blotting, immunofluorescence, or immunoprecipitation. Studies using this antibody have advanced understanding of replication stress resolution, ATR/ATRIP signaling dynamics, and potential therapeutic targets in diseases involving defective DDR, such as cancers or neurodegenerative disorders. Specificity is typically confirmed using phosphorylation-deficient mutants or phosphatase treatments.