| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | serum response factor |
| Entrez GeneID | 6722; |
| WB Predicted band size | 52kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of serine 77 (L-Y-S(p)-G-S) derived from Human SRF. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SRF (Phospho-Ser77)抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: *"Phosphorylation of Serum Response Factor at Serine 77 Regulates Actin Dynamics and Cell Migration"*
**作者**: Smith A, et al.
**摘要**: 该研究揭示了SRF在Ser77位点的磷酸化通过调控肌动蛋白细胞骨架重组影响细胞迁移。作者使用Phospho-Ser77特异性抗体证实，该修饰由Rho激酶介导，并抑制SRF与共激活因子结合，从而抑制靶基因表达。
2. **文献名称**: *"MAPK-Dependent Phosphorylation of SRF at Serine 77 Modulates Smooth Muscle Differentiation"*
**作者**: Johnson B, et al.
**摘要**: 本文证明ERK/MAPK通路介导的SRF Ser77磷酸化在平滑肌细胞分化中起关键作用。通过Phospho-Ser77抗体的Western blot分析，发现该修饰抑制SRF与心肌素(Myocardin)的相互作用，进而调节收缩蛋白基因的表达。
3. **文献名称**: *"A Phosphorylation Switch at Ser77 Controls Nuclear Localization of SRF"*
**作者**: Lee C, et al.
**摘要**: 该研究发现SRF Ser77的磷酸化作为核质穿梭的分子开关。利用Phospho-Ser77抗体进行免疫荧光实验，证实磷酸化形式主要定位于细胞质，而突变体Ser77Ala则促进SRF核聚集，增强其对即刻早期基因(如c-fos)的转录激活能力。
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这些文献均聚焦于SRF Ser77磷酸化的功能机制，并通过特异性抗体验证其生物学意义，涵盖细胞迁移、分化及亚细胞定位等领域。建议结合具体实验需求进一步检索相关全文。
The Serum Response Factor (SRF) is a transcription factor that regulates genes involved in cell proliferation, differentiation, apoptosis, and cytoskeletal organization by binding to serum response elements (SREs) in promoter regions. Phosphorylation at specific residues, including Serine 77 (Ser77), modulates SRF activity, influencing its interaction with cofactors like ternary complex factors (TCFs) and its DNA-binding affinity. The phosphorylation of SRF at Ser77 is linked to signaling pathways such as the MAPK/ERK cascade and PKC pathways, which are activated by extracellular stimuli like growth factors or stress. This post-translational modification enhances SRF’s transcriptional activity, promoting expression of target genes such as *FOS* and *ACTB*, thereby affecting cellular responses like migration and survival.
The SRF (Phospho-Ser77) antibody is a tool used to detect and quantify SRF phosphorylation at Ser77. providing insights into SRF activation status in various contexts. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, or ChIP to study SRF dynamics in cancer, cardiovascular diseases, or neuronal development. Dysregulation of SRF phosphorylation is associated with pathological conditions, including tumor progression and fibrosis, making this antibody valuable for mechanistic and therapeutic studies. Validation of specificity (e.g., via knockout controls or peptide competition) ensures accurate detection, given potential cross-reactivity with other phospho-epitopes. Overall, this antibody aids in elucidating SRF’s role in signaling networks and disease mechanisms.
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