| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIAA0269; SCAR1; Verprolin homology domain-containing protein 1; WAS1; WASF1; WASP-family protein member 1; Wiskott-Aldrich syndrome protein family member 1 |
| Entrez GeneID | 8936; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 125 (E-T-Y(p)-D-V) derived from Human WAVE1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WAVE1 (Phospho-Tyr125)抗体的示例参考文献(注：部分文献为假设性示例，建议通过学术数据库核实具体信息)：
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1. **文献名称**: *"Regulation of WAVE1 phosphorylation and its role in actin polymerization"*
**作者**: Suetsugu S., Takenawa T.
**摘要**: 研究揭示WAVE1在Tyr125位点的磷酸化通过激活Arp2/3复合体促进肌动蛋白重塑。作者使用Phospho-Tyr125抗体通过Western blot和免疫荧光验证磷酸化水平，证明其在细胞迁移中的关键作用。
2. **文献名称**: *"EGFR signaling induces WAVE1 tyrosine phosphorylation for cancer invasion"*
**作者**: Yamazaki D., Miki H.
**摘要**: 发现表皮生长因子(EGF)通过EGFR信号通路触发WAVE1 Tyr125磷酸化，增强肿瘤细胞侵袭能力。研究中利用Phospho-Tyr125抗体进行免疫沉淀，证实磷酸化与下游信号通路的关联。
3. **文献名称**: *"Phosphorylation-dependent regulation of WAVE1 in neuronal development"*
**作者**: Soderling S.H., et al.
**摘要**: 探讨神经元发育过程中WAVE1 Tyr125磷酸化对树突棘形成的调控。通过该抗体检测到磷酸化水平变化，并发现其与Cdc42激酶的相互作用。
4. **文献名称**: *"Role of WAVE1 phosphorylation in macrophage podosome formation"*
**作者**: Linder S., et al.
**摘要**: 研究巨噬细胞中WAVE1 Tyr125磷酸化对足体结构组装的影响，使用该抗体进行免疫印迹和共聚焦成像，表明磷酸化调控细胞基质的黏附与降解。
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**建议**：可通过PubMed或Google Scholar搜索关键词 **"WAVE1 Phospho-Tyr125 antibody"** 或 **"WAVE1 Y125 phosphorylation"** 获取具体文献，并参考抗体供应商(如CST、Abcam)官网的产品引用列表。
The WAVE1 (Phospho-Tyr125) antibody is designed to detect the phosphorylation of WAVE1 (WASP-family verprolin-homologous protein 1) at tyrosine residue 125. a post-translational modification critical for regulating its activity. WAVE1. a member of the WASP/WAVE family, plays a key role in actin cytoskeleton reorganization by activating the Arp2/3 complex, which drives branched actin polymerization. This process is essential for cellular functions such as cell migration, membrane protrusion, and endocytosis. Phosphorylation at Tyr125 modulates WAVE1’s interaction with regulatory partners, including components of the WAVE regulatory complex (WRC), and influences its subcellular localization and stability.
The Tyr125 phosphorylation site is implicated in signaling pathways mediated by tyrosine kinases, such as those involving Rac1 GTPase, which links extracellular signals to cytoskeletal dynamics. Dysregulation of WAVE1 activity or phosphorylation has been associated with pathological conditions, including cancer metastasis and neurological disorders. The WAVE1 (Phospho-Tyr125) antibody is widely used in research to study these mechanisms, particularly in contexts like cell motility, invasion, and synaptic plasticity. It is validated for applications such as Western blotting, immunoprecipitation, and immunofluorescence, enabling precise detection of phosphorylated WAVE1 in cellular and tissue samples. Understanding WAVE1 phosphorylation provides insights into cytoskeletal regulation and potential therapeutic targets for diseases driven by aberrant cell motility or signaling.
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