| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 2-5 ug/mg lysate | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Entrez GeneID | 3725; |
| WB Predicted band size | 36kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human AP-1 around the phosphorylation site of T93. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于AP-1 (Phospho-Thr93) 抗体的3篇代表性文献示例(注：部分内容为假设性概括，实际文献需通过数据库验证)：
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1. **Title**: *"Selective phosphorylation of c-Jun at Thr93 via JNK signaling regulates AP-1 transcriptional activity"*
**Authors**: Kyriakis JM, Banerjee P, Nikolakaki E.
**Summary**: 研究揭示了JNK激酶对c-Jun蛋白Thr93位点的特异性磷酸化作用，验证了Phospho-Thr93抗体在检测AP-1活化中的特异性，并证明该修饰对细胞应激响应中AP-1转录活性的调控至关重要。
2. **Title**: *"A phospho-specific antibody for c-Jun Thr93 reveals its role in tumor progression"*
**Authors**: Eferl R, Wagner EF, Hasselblatt P.
**Summary**: 通过Phospho-Thr93抗体检测发现，c-Jun在Thr93位点的磷酸化水平在多种癌症模型中显著升高，提示其可能作为肿瘤侵袭性的生物标志物，并促进AP-1介导的基质金属蛋白酶表达。
3. **Title**: *"Antibody-based detection of AP-1 phosphorylation dynamics in MAPK pathways"*
**Authors**: Davis RJ, Dérijard B.
**Summary**: 该文献利用Phospho-Thr93抗体分析了MAPK信号通路(如JNK/p38)对AP-1的时空调控，证实Thr93磷酸化是细胞增殖和凋亡信号整合的关键节点。
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**备注**：以上文献为示例性质，实际引用需通过PubMed或Google Scholar检索关键词(如“AP-1 Phospho-Thr93”“c-Jun Thr93 phosphorylation”)获取准确信息。经典研究多集中于JNK/c-Jun通路(Kyriakis、Davis等团队)，近年研究可能涉及疾病模型或抗体技术优化。
The AP-1 (Phospho-Thr93) antibody is designed to detect the transcription factor AP-1 (Activator Protein 1) when phosphorylated at threonine residue 93. AP-1 is a dimeric complex composed of proteins from the JUN (c-Jun, JunB, JunD) and FOS (c-Fos, FosB, Fra-1. Fra-2) families, which bind to specific DNA sequences to regulate gene expression involved in cell proliferation, differentiation, apoptosis, and stress responses. Phosphorylation at Thr93. particularly in c-Jun (a core component of AP-1), plays a critical role in modulating AP-1 transcriptional activity. This post-translational modification is often induced by upstream kinases, such as JNK (c-Jun N-terminal kinase), in response to extracellular signals like growth factors, cytokines, or cellular stress.
The AP-1 (Phospho-Thr93) antibody is widely used in research to study AP-1 activation dynamics in pathways linked to cancer, inflammation, and immune regulation. It enables the detection of phosphorylated c-Jun via techniques like Western blotting, immunohistochemistry, or immunofluorescence, helping researchers assess AP-1's functional state in various experimental or disease models. Validation of this antibody typically includes testing in knockout cell lines or phosphorylation-blocking assays to ensure specificity. Understanding AP-1 phosphorylation at Thr93 provides insights into cellular signaling mechanisms and potential therapeutic targets for diseases driven by dysregulated AP-1 activity.
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