| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CEBPB; LAP; TCF5; PP9092; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5 |
| Entrez GeneID | 1051; |
| WB Predicted band size | 36kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human C/EBP β around the phosphorylation site of T235. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与C/EBP β (Phospho-Thr235)抗体相关的参考文献(虚拟示例，非真实文献)：
1. **"Phosphorylation of C/EBPβ at Thr235 regulates macrophage differentiation"**
- Author: Smith J, et al.
- 摘要：该研究通过Phospho-Thr235抗体证实，C/EBPβ在Thr235位点的磷酸化是巨噬细胞分化中IL-6信号通路激活的关键步骤，并影响靶基因转录调控。
2. **"C/EBPβ phosphorylation controls lipid metabolism in adipocytes"**
- Author: Lee H, et al.
- 摘要：利用Phospho-Thr235特异性抗体，作者发现小鼠脂肪细胞中C/EBPβ的Thr235磷酸化通过mTOR通路调控脂质合成相关基因表达，影响肥胖进程。
3. **"Role of C/EBPβ Thr235 phosphorylation in LPS-induced inflammation"**
- Author: Chen R, et al.
- 摘要：通过Western blot和免疫荧光技术，研究证明LPS刺激下巨噬细胞C/EBPβ Thr235磷酸化水平显著升高，该修饰通过NF-κB通路增强炎症因子释放。
注：以上文献为模拟内容，实际引用需检索PubMed等数据库获取真实论文，推荐使用关键词"C/EBPβ Phospho-Thr235"或"CEBPB Thr235 phosphorylation"查找实验验证型研究。
The C/EBP β (Phospho-Thr235) antibody is a specialized tool used to detect the phosphorylated form of the CCAAT/enhancer-binding protein beta (C/EBP β) at threonine 235. a post-translational modification critical for regulating its activity. C/EBP β is a transcription factor involved in cellular processes such as differentiation, proliferation, inflammation, and metabolism. Phosphorylation at Thr235. located within its regulatory domain, modulates C/EBP β’s transcriptional activity by altering its DNA-binding affinity or interaction with co-regulators. This modification is often induced by signaling pathways like MAPK or CDK, linking extracellular signals to gene expression changes.
Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study the activation status of C/EBP β in response to stimuli such as cytokines, hormones, or stress. It is particularly valuable in investigating conditions where C/EBP β phosphorylation plays a role, including cancer progression, immune responses, adipogenesis, and liver regeneration. For example, hyperphosphorylation at Thr235 has been associated with tumorigenesis or inflammatory diseases, making this antibody a key reagent for mechanistic studies. Its specificity for the phosphorylated epitope ensures accurate detection of the active form, aiding in the exploration of C/EBP β’s context-dependent functions in health and disease. Proper validation using knockout controls or phosphatase treatment is essential to confirm signal specificity.
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