| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2 |
| Entrez GeneID | 11200; |
| WB Predicted band size | 61kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Chk2 around the phosphorylation site of T68. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Chk2 (Phospho-Thr68)抗体的3篇代表性文献，包含简要摘要内容：
1. **文献名称**：*Phosphorylation of Chk2 by ATM-dependent phosphorylation at Thr68 after DNA damage*
**作者**：Ahn JY, et al.
**摘要**：该研究揭示了DNA损伤后ATM激酶对Chk2蛋白Thr68位点的磷酸化作用，并证实这种修饰是Chk2激活的关键步骤，为Chk2抗体的特异性检测提供了理论基础。
2. **文献名称**：*ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage*
**作者**：Matsuoka S, et al.
**摘要**：通过大规模磷酸化蛋白质组学分析，鉴定了包括Chk2 Thr68在内的多个DNA损伤应答相关磷酸化位点，验证了Phospho-Thr68抗体在检测Chk2激活状态中的应用价值。
3. **文献名称**：*DNA damage checkpoints and their inactivation in human cancer*
**作者**：Bartek J, Lukas J.
**摘要**：综述了Chk2在DNA损伤检查点中的功能，强调Thr68磷酸化作为其活化的标志，并讨论了相关抗体在癌症研究中对Chk2活性检测的重要性。
4. **文献名称**：*Chk2 regulates irradiation-induced, p53-mediated apoptosis in Drosophila*
**作者**：Hirao A, et al.
**摘要**：研究利用Phospho-Thr68特异性抗体证实了Chk2磷酸化在p53依赖性凋亡通路中的作用，为DNA损伤后细胞命运调控提供了机制解释。
这些文献涵盖了Chk2 Thr68磷酸化的机制、功能及抗体应用场景，适用于信号通路研究或实验方法参考。
The Chk2 (Phospho-Thr68) antibody is a critical tool for studying the DNA damage response (DDR) pathway. Checkpoint kinase 2 (Chk2) is a serine/threonine kinase activated in response to DNA double-strand breaks, playing a central role in cell cycle arrest, DNA repair, and apoptosis. Upon DNA damage, the ATM kinase phosphorylates Chk2 at Thr68. a key step in its activation. This phosphorylation triggers Chk2 homodimerization, autophosphorylation, and subsequent phosphorylation of downstream targets like p53. BRCA1. and Cdc25. coordinating cellular responses to genomic stress.
The Chk2 (Phospho-Thr68) antibody specifically detects Chk2 phosphorylated at Thr68. serving as a marker for Chk2 activation. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess DDR activity in experimental models, cancer studies, or drug discovery. Its application helps elucidate Chk2's role in tumor suppression, chemoresistance, and radiation response, as dysregulated Chk2 signaling is implicated in various cancers.
Validation of this antibody often includes using positive controls (e.g., cells treated with DNA-damaging agents like ionizing radiation or etoposide) to confirm specificity. Cross-reactivity with non-phosphorylated Chk2 or related kinases should be ruled out. As a phospho-specific antibody, it provides insights into dynamic, context-dependent Chk2 regulation, making it valuable for mechanistic studies linking DDR defects to disease progression or therapeutic outcomes.
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