Rabbit Polyclonal Hrs(Phospho-Tyr216) Antibody

The Hrs (Phospho-Tyr216) antibody detects the phosphorylated form of hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) at tyrosine residue 216. Hrs, a key component of the endosomal sorting complex required for transport (ESCRT), plays a critical role in endocytic trafficking and lysosomal degradation of ubiquitinated membrane proteins. Phosphorylation at Tyr216. mediated by Src family kinases or other tyrosine kinases, regulates Hrs' interaction with downstream effectors like STAM (signal-transducing adaptor molecule) and ESCRT-I components, facilitating cargo sorting into multivesicular bodies (MVBs). This post-translational modification is crucial for modulating endosome maturation, receptor downregulation (e.g., EGFR), and autophagic processes. The Hrs (Phospho-Tyr216) antibody is widely used in studies investigating membrane trafficking dynamics, receptor signaling termination, and diseases linked to lysosomal dysfunction, such as cancer and neurodegenerative disorders. Specificity is typically validated via peptide-blocking assays or using phosphorylation-deficient mutants. Applications include Western blotting, immunofluorescence, and immunohistochemistry to assess Hrs activation status in cell lines or tissue samples under varying experimental conditions.