The IRS-1 (Phospho-Ser794) antibody is a specialized tool used to detect insulin receptor substrate 1 (IRS-1) when phosphorylated at serine residue 794. IRS-1 is a key adaptor protein in insulin and insulin-like growth factor (IGF) signaling pathways, mediating downstream effects on glucose uptake, metabolism, and cell growth. Phosphorylation at specific serine residues, including Ser794. regulates IRS-1 activity and downstream signaling. Ser794 phosphorylation is associated with negative feedback mechanisms, often triggered by hyperactivation of pathways like mTOR or stress kinases, leading to IRS-1 degradation or impaired insulin signaling. This post-translational modification is implicated in insulin resistance, a hallmark of metabolic disorders such as type 2 diabetes and obesity. The IRS-1 (Phospho-Ser794) antibody enables researchers to study the dynamic regulation of IRS-1 in response to insulin, growth factors, or cellular stressors, using techniques like Western blotting, immunohistochemistry, or immunofluorescence. Its application extends to investigating molecular mechanisms underlying metabolic diseases, drug responses, and the interplay between IRS-1 and kinases like Akt, mTOR, or JNK. Validated specificity for the phosphorylated epitope ensures accurate detection of this regulatory modification in experimental models.
