| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1D |
| Uniprot No | Q13901 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-141aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAGEEIN EDYPVEIHEY LSAFENSIGA VDEMLKTMMS VSRNELLQKL DPLEQAKVDL VSAYTLNSMF WVYLATQGVN PKEHPVKQEL ERIRVYMNRV KEITDKKKAG KLDRGAASRF VKNALWEPKS KNASKVANKG KSKS |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C1D重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Cloning and functional characterization of C1D, a novel human nuclear protein involved in RNA processing and apoptosis"*
**作者**: Smith A, et al.
**摘要**: 本研究克隆并表达了人源C1D重组蛋白，发现其通过与RNA结合蛋白相互作用参与RNA剪接和细胞凋亡调控，揭示了C1D在DNA损伤应答中的潜在作用。
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2. **文献名称**: *"Structural and biochemical analysis of recombinant C1D in non-homologous end joining (NHEJ) pathway"*
**作者**: Johnson R, et al.
**摘要**: 通过重组C1D蛋白的晶体结构解析和体外生化实验，证明C1D通过与DNA-PK复合物结合促进非同源末端连接(NHEJ)修复，为理解其维持基因组稳定的机制提供依据。
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3. **文献名称**: *"C1D interacts with XRN2 and modulates its activity in ribosomal RNA biogenesis"*
**作者**: Lee S, et al.
**摘要**: 研究利用重组C1D蛋白进行免疫共沉淀实验，发现C1D与核酸外切酶XRN2相互作用，调控rRNA加工过程，提示其在核糖体合成中的关键功能。
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(注：以上文献为示例性内容，实际研究中请根据具体论文调整信息。)
**Background of C1D Recombinant Protein**
C1D (also known as *C1D nuclear receptor corepressor* or *p28*) is a multifunctional protein involved in critical cellular processes, including DNA repair, RNA metabolism, and transcriptional regulation. It was initially identified as a co-repressor for nuclear hormone receptors but later found to interact with components of the non-homologous end-joining (NHEJ) pathway, a key DNA double-strand break repair mechanism. Structurally, C1D contains an N-terminal leucine-rich region and a C-terminal acidic domain, facilitating interactions with proteins like DNA-PKcs (DNA-dependent protein kinase catalytic subunit) and RNA-processing factors.
Recombinant C1D protein is produced via genetic engineering, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. Purification methods often employ affinity tags (e.g., His-tag) for high yield and purity. Its recombinant form enables detailed biochemical and structural studies, such as elucidating its role in stabilizing DNA-PK complexes during repair or its involvement in RNA splicing via interactions with small nuclear ribonucleoproteins (snRNPs).
C1D's relevance extends to disease research, particularly cancer and autoimmune disorders. Dysregulation of C1D has been linked to genomic instability and impaired immune responses. Additionally, recombinant C1D serves as a tool for drug screening, aiming to target pathways involving DNA repair or transcriptional regulation. Recent studies also explore its potential in gene therapy and as a biomarker for therapeutic resistance.
Overall, recombinant C1D protein provides a versatile platform for dissecting molecular mechanisms in genome maintenance and cellular signaling, with implications for both basic research and translational applications.
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