| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 1/100-1/300 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 1/5000 | Rat |
| Aliases | TH; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Synthesized peptide derived from human TH around the phosphorylation site of S8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于TH (Phospho-Ser8)抗体的3篇示例文献(内容为模拟概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**: "Phosphorylation of tyrosine hydroxylase at serine 8 modulates enzyme activity and dopamine biosynthesis"
**作者**: Haycock JW, et al.
**摘要**: 研究揭示了酪氨酸羟化酶(TH)在Ser8位点的磷酸化对其催化活性的调控机制，通过体外酶活性实验证明磷酸化可增强TH与辅因子的结合能力，从而促进多巴胺合成。
2. **文献名称**: "Dopaminergic neuron-specific phosphorylation of tyrosine hydroxylase at Ser8 in response to oxidative stress"
**作者**: Lew JY, et al.
**摘要**: 在帕金森病细胞模型中，发现氧化应激通过激活特定激酶(如MAPK)诱导TH-Ser8磷酸化，导致酶活性代偿性升高，可能是一种神经保护机制。
3. **文献名称**: "Differential phosphorylation of tyrosine hydroxylase isoforms at Ser8 and Ser40 in adrenal chromaffin cells"
**作者**: Dunkley PR, Bobrovskaya L.
**摘要**: 比较肾上腺髓质嗜铬细胞中TH不同亚型的磷酸化模式，发现Ser8磷酸化主要受Ca²⁺信号通路调控，而Ser40磷酸化与cAMP通路相关，提示不同位点磷酸化的功能分工。
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**说明**：
- 以上文献为示例，实际研究中建议通过PubMed、Google Scholar等平台检索关键词(如“tyrosine hydroxylase phosphorylation Ser8”)获取最新或经典文献。
- TH的磷酸化研究常涉及激酶通路(如PKA、MAPK)、疾病模型(帕金森病、应激反应)及酶动力学分析。
The TH (Phospho-Ser8) antibody is a specialized tool used to study the phosphorylation state of tyrosine hydroxylase (TH), a rate-limiting enzyme in catecholamine biosynthesis. TH catalyzes the conversion of L-tyrosine to L-DOPA, a precursor of dopamine, norepinephrine, and epinephrine. Its activity is tightly regulated by phosphorylation at specific serine residues, including Ser8. Ser19. Ser31. and Ser40. Phosphorylation at Ser8. though less characterized than other sites, is implicated in modulating TH interactions with regulatory proteins or influencing enzyme stability under certain cellular conditions.
This antibody specifically recognizes TH when phosphorylated at serine 8. enabling researchers to investigate post-translational modifications linked to TH regulation. Such modifications may reflect cellular responses to stimuli like stress, neuronal activity, or pharmacological agents. Phospho-Ser8 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, or immunofluorescence to map TH phosphorylation dynamics in dopaminergic neurons, adrenal glands, or neuroendocrine cells.
Studies utilizing this antibody contribute to understanding disorders involving catecholamine dysregulation, such as Parkinson’s disease, depression, or hypertension. By detecting Ser8 phosphorylation, researchers can explore how TH activity adapts to physiological or pathological states, offering insights into therapeutic strategies targeting catecholaminergic pathways. Validation typically includes testing in knockout models or phosphatase-treated samples to confirm specificity for the phosphorylated epitope.
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