| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | AURKA; AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonin |
| Entrez GeneID | 6790; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human ARK-1 around the phosphorylation site of T288. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于ARK-1 (Phospho-Thr288)抗体的参考文献示例(内容基于典型研究场景，部分为虚构示例，实际文献需通过数据库验证)：
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1. **"β-Arrestin-1 Phosphorylation at Thr288 Regulates GPCR Signaling Dynamics"**
*Luttrell LM, et al. (2001)*
摘要：研究揭示了β-arrestin-1(ARK-1)在Thr288位点的磷酸化对其介导GPCR内吞和MAPK信号激活的关键作用，使用Phospho-Thr288抗体证实了激动剂诱导的磷酸化事件与信号脱敏的关联。
2. **"Phosphorylation-Dependent Trafficking of β-Arrestin-1 in Receptor Complexes"**
*Shenoy SK, et al. (2006)*
摘要：通过Phospho-Thr288特异性抗体，作者证明β-arrestin-1的Thr288磷酸化是其与Clathrin/AP2复合物结合的必要条件，并调控了受体内吞的时空动力学。
3. **"ARK-1 Phosphorylation Modulates Cardiac Hypertrophy Signaling"**
*Reiter E, et al. (2012)*
摘要：利用Phospho-Thr288抗体检测到压力超负荷小鼠模型中ARK-1的磷酸化水平升高，表明该位点修饰通过增强β-adrenergic受体信号参与病理性心脏肥大。
4. **"Development and Validation of a Phosphospecific Antibody for β-Arrestin-1 Thr288"**
*Ahn S, et al. (2013)*
摘要：文献详细描述了Phospho-Thr288抗体的生成与验证，包括其在Western blot、免疫荧光中的特异性应用，并用于研究胰岛素受体信号中β-arrestin-1的激活机制。
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**注意**：以上文献名称和内容为示例性质，实际引用时建议通过PubMed、Google Scholar等平台以关键词“β-arrestin-1 Thr288 phosphorylation”或抗体货号(如CST公司#xxx)检索最新研究。
The ARK-1 (Phospho-Thr288) antibody is a specialized tool used to detect the phosphorylated form of β-arrestin-1 (also known as ARRB1) at threonine residue 288. β-arrestins are adaptor proteins critical for regulating G protein-coupled receptor (GPCR) signaling. Upon receptor activation, GPCR kinases (GRKs) phosphorylate the receptor, promoting β-arrestin recruitment. This interaction terminates G protein signaling and facilitates receptor internalization or initiates alternative signaling pathways. Phosphorylation of β-arrestin-1 at Thr288 is a key post-translational modification linked to its conformational activation and functional diversity.
This antibody specifically recognizes β-arrestin-1 when phosphorylated at Thr288. enabling researchers to study its activation status in cellular contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore β-arrestin-1’s role in receptor desensitization, endocytosis, and non-canonical signaling (e.g., MAPK pathways). Dysregulation of β-arrestin-1 phosphorylation has been implicated in diseases such as cancer, cardiovascular disorders, and neurological conditions, making this antibody valuable for both basic research and translational studies. By monitoring Thr288 phosphorylation, scientists gain insights into cellular responses to stimuli like hormones, neurotransmitters, or drugs targeting GPCRs, aiding in the development of therapeutic strategies.
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