| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu |
| Entrez GeneID | 5587; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human PKD1 around the phosphorylation site of S205. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PKD1 (Phospho-Ser205)抗体的模拟参考文献示例(注：部分内容为基于领域知识的模拟，建议通过学术数据库核实具体文献)：
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1. **Title**: *Phosphorylation of PKD1 at Ser205 regulates kinase activity in cardiac hypertrophy*
**Authors**: Smith, J.R., et al.
**摘要**: 本研究通过构建Ser205磷酸化位点突变体，发现PKD1在心肌细胞肥大中的激活机制。使用Phospho-Ser205特异性抗体进行免疫印迹，证实血管紧张素II刺激后该位点的磷酸化水平升高，且与病理型心脏重塑相关。
2. **Title**: *PKD1 Ser205 phosphorylation mediates inflammatory signaling in macrophages*
**Authors**: Chen, L., et al.
**摘要**: 研究报道LPS通过TLR4通路诱导PKD1 Ser205磷酸化，促进NF-κB活化。采用Phospho-Ser205抗体进行免疫荧光染色，显示磷酸化PKD1在炎症小体形成中的亚细胞定位变化。
3. **Title**: *Development of a phospho-specific antibody for PKD1 Ser205 and its application in cancer models*
**Authors**: Wang, Y., et al.
**摘要**: 本文详细描述了Phospho-Ser205抗体的制备与验证(包括肽段竞争实验)。应用该抗体发现，在结直肠癌组织中PKD1 Ser205磷酸化水平与肿瘤侵袭性正相关，提示其作为预后标志物的潜力。
4. **Title**: *PKD1 activation loop phosphorylation at Ser205 is required for Golgi fragmentation during mitosis*
**Authors**: Ramirez, S., et al.
**摘要**: 利用Phospho-Ser205抗体及活细胞成像技术，证明有丝分裂中PKD1在Ser205位点的磷酸化由PLK1激酶介导，并调控高尔基体分解与细胞周期进程的协调。
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建议通过**PubMed**或**Google Scholar**以关键词“PKD1 Ser205 phosphorylation antibody”检索最新文献，重点关注《Journal of Biological Chemistry》《Cell Signaling》等期刊的相关研究。
The PKD1 (Phospho-Ser205) antibody is a specialized tool used to detect the activated form of Protein Kinase D1 (PKD1), a serine/threonine kinase involved in diverse cellular processes, including proliferation, apoptosis, migration, and stress responses. PKD1 activation typically occurs via phosphorylation at specific residues, such as Ser205. which lies within its activation loop. Phosphorylation at Ser205 is critical for PKD1’s catalytic activity, often triggered by upstream signals like G protein-coupled receptors (GPCRs) or growth factor receptors. This antibody specifically recognizes PKD1 when phosphorylated at Ser205. enabling researchers to study its activation dynamics in various contexts.
The antibody is widely used in Western blotting, immunofluorescence, and immunoprecipitation to investigate PKD1 signaling in diseases such as cancer, cardiovascular disorders, and inflammation. In cancer research, PKD1 activity has been linked to tumor progression, metastasis, and resistance to therapy, with phosphorylation at Ser205 serving as a biomarker for pathway activation. Validations often include testing in cell lines with PKD1 knockdown or pharmacological inhibitors to confirm specificity. Its role in regulating NF-κB, ERK, and other downstream effectors underscores its relevance in both basic research and therapeutic targeting. Proper controls, such as λ-phosphatase treatment, are recommended to ensure accurate interpretation of phosphorylation status.
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