| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | STK3; KRS1; MST2; Serine/threonine-protein kinase 3; Mammalian STE20-like protein kinase 2; MST-2; STE20-like kinase MST2; Serine/threonine-protein kinase Krs-1; STK4; KRS2; MST1; Serine/threonine-protein kinase 4; Mammalian STE20-like prot |
| Entrez GeneID | 6788; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human Krs-1/2 around the phosphorylation site of T183. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **KSR1/2 (Phospho-Thr183)** 抗体的参考文献示例(内容为模拟生成，仅供参考)：
1. **文献名称**：*Phosphorylation of KSR1 at Thr183 Promotes ERK Activation by Facilitating MEK Binding*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了KSR1在Thr183位点的磷酸化通过增强其与MEK的相互作用促进RAS/MAPK信号通路的激活，该抗体用于检测细胞中KSR1的磷酸化状态及其在肿瘤发生中的作用。
2. **文献名称**：*Dynamic Regulation of KSR1 Phosphorylation Controls MAPK Signaling Output*
**作者**：McKay MM, et al.
**摘要**：通过Phospho-Thr183抗体验证KSR1在生长因子刺激下的磷酸化动态变化，证明该位点磷酸化是KSR1从胞质向膜转位的关键调控步骤。
3. **文献名称**：*A Phosphospecific Antibody Reveals KSR2 Thr183 Phosphorylation in Metabolic Regulation*
**作者**：Costanzo-Garvey DL, et al.
**摘要**：开发了一种特异性识别KSR2 Thr183磷酸化位点的抗体，并发现该位点的磷酸化参与调控胰岛素信号通路和能量代谢平衡。
4. **文献名称**：*Targeting KSR1 Phosphorylation for Therapeutic Intervention in RAS-Driven Cancers*
**作者**：Lozano J, et al.
**摘要**：利用Phospho-Thr183抗体筛选小分子抑制剂，发现抑制KSR1磷酸化可有效阻断RAS突变型肿瘤细胞的增殖和存活。
**注**：以上文献为示例，实际引用时需通过PubMed、Google Scholar等平台检索真实文献(关键词：KSR1/2. Phospho-Thr183. MAPK signaling)。
The Ksr-1/2 (Phospho-Thr183) antibody is designed to detect the phosphorylated form of Kinase Suppressor of Ras 1 and 2 (KSR1/2) at threonine 183. a post-translational modification critical for regulating their scaffolding function in the Ras/Raf/MEK/ERK signaling pathway. KSR1 and KSR2 are pseudokinases that act as molecular scaffolds to facilitate signal transduction between Ras, Raf, MEK, and ERK kinases, playing a key role in cell proliferation, differentiation, and survival. Phosphorylation at Thr183 in KSR1 (or the corresponding site in KSR2) is associated with conformational changes that enhance its interaction with downstream components like Raf and MEK, promoting ERK activation. This modification is often triggered by growth factor signaling or oncogenic stimuli, making it a marker of pathway activity.
The Phospho-Thr183 antibody is widely used in research to study the activation status of the Ras/MAPK pathway in cancer, developmental biology, and cellular responses to targeted therapies. It enables detection of phosphorylated KSR1/2 via techniques such as Western blotting, immunofluorescence, or immunoprecipitation, helping to elucidate mechanisms of drug resistance or oncogenic signaling. Specific validation for cross-reactivity and phosphorylation-dependent signal specificity is essential, as dysregulation of KSR phosphorylation is linked to tumor progression and therapeutic outcomes. This antibody serves as a valuable tool for probing Ras pathway dynamics in both physiological and disease contexts.
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