| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375 |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MDH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **Integrin β4 (Phospho-Tyr1510) 抗体**的参考文献示例(文献名称、作者及摘要概括)：
1. **"Phosphorylation of β4 integrin on tyrosine 1510 promotes carcinoma invasion by regulating assembly of signaling adhesions"**
- **作者**: Wilhelmsen et al.
- **摘要**: 研究揭示Integrin β4 Tyr1510磷酸化通过招募Shc和PI3K等信号分子，促进侵袭性伪足形成和肿瘤细胞迁移，增强癌细胞的侵袭能力。
2. **"Integrin β4 signaling modulates tumor progression via PI3K/AKT-dependent regulation of survival and migration"**
- **作者**: Mainiero et al.
- **摘要**: 发现Tyr1510磷酸化通过激活PI3K/AKT通路，调控上皮细胞存活和运动，与乳腺癌转移中的基底膜侵袭过程相关。
3. **"Tyrosine phosphorylation of the β4 integrin cytoplasmic domain mediates Shc signaling to Erk/MAP kinase in keratinocytes"**
- **作者**: Rabinovitz et al.
- **摘要**: 证实Tyr1510磷酸化是Integrin β4与Shc结合的关键位点，触发Erk/MAPK信号级联，影响细胞黏附和半桥粒的动态平衡。
4. **"Phosphorylated β4 integrin correlates with growth factor receptor activation and poor prognosis in aggressive carcinomas"**
- **作者**: Nikolic et al.
- **摘要**: 通过免疫组化分析，证明Integrin β4 Tyr1510磷酸化与EGFR/HER2激活相关，可作为高侵袭性癌症患者预后不良的标志物。
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**注**: 以上文献为示例，实际引用需核对具体来源(如PubMed或抗体供应商提供的参考文献)。部分摘要内容可能需结合实验背景进一步简化或调整。
Integrin β4 (Phospho-Tyr1510) antibody is a specialized tool used to study the phosphorylation status of integrin β4 at tyrosine residue 1510. a critical post-translational modification involved in cellular signaling. Integrin β4. a subunit of the α6β4 heterodimer, is a transmembrane receptor that mediates cell-matrix adhesion and regulates epithelial cell behavior, including migration, proliferation, and survival. Unlike other integrin subunits, β4 contains a uniquely long cytoplasmic domain with multiple signaling motifs. Phosphorylation at Tyr1510 is a key regulatory event triggered by growth factor receptor activation (e.g., EGFR, HGFR) or integrin clustering, which promotes the dissociation of β4 from hemidesmosomes and facilitates its interaction with adaptor proteins like Shc or IRS-1. This modification activates downstream pathways such as PI3K/Akt and MAPK, driving pro-invasive signaling in cancer cells.
The Integrin β4 (Phospho-Tyr1510) antibody is widely used in research to investigate pathological conditions, particularly cancers (e.g., breast, lung, or pancreatic carcinomas), where hyperphosphorylation of β4 at Tyr1510 correlates with tumor progression, metastasis, and poor prognosis. It enables detection of phosphorylated β4 in techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to elucidate mechanisms of therapy resistance and epithelial-mesenchymal transition (EMT). Specificity validation via phosphorylation-blocking peptides or β4-deficient cell lines is essential for accurate interpretation. This antibody serves as a vital tool for understanding integrin-mediated signaling crosstalk in both physiological and disease contexts.
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