Rabbit Polyclonal Caspase-3(Phospho-Ser150) Antibody

中文名： Caspase-3 (Phospho-Ser150)抗体
别名： CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

货号: IPDX40919

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/300	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	1/40000	Human,Mouse,Rat

产品详情

Aliases	CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Entrez GeneID	836;
WB Predicted band size	34kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Caspase-3 around the phosphorylation site of S150.
Formulation	Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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Western blot analysis of HBE using p-Caspase-3 (S150) antibody.

参考文献

以下是关于Caspase-3 (Phospho-Ser150)抗体的3篇模拟参考文献示例(注：文献为虚构，仅供格式参考)：

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1. **文献名称**: *Phosphorylation of Caspase-3 at Ser150 modulates its activation in DNA damage-induced apoptosis*

**作者**: Zhang, Y. et al.

**摘要**: 本研究通过免疫沉淀和Western blot分析，证实DNA损伤剂诱导的Caspase-3 Ser150磷酸化可抑制其酶活性，延缓细胞凋亡进程。该抗体特异性识别磷酸化位点，为研究凋亡调控提供了工具。

2. **文献名称**: *A novel role of Caspase-3 pSer150 in neuronal survival under oxidative stress*

**作者**: Smith, J.R. et al.

**摘要**: 在神经元模型中，利用Caspase-3 (Phospho-Ser150)抗体发现，氧化应激通过ERK通路触发Ser150磷酸化，阻断Caspase-3切割，提示该位点可能参与神经退行性疾病的保护机制。

3. **文献名称**: *Development and validation of a phospho-specific antibody for Caspase-3 Ser150 in breast cancer models*

**作者**: Lee, H. et al.

**摘要**: 报道了一种高特异性抗体的开发，通过CRISPR/Cas9敲入突变细胞系验证其仅识别Ser150磷酸化形式。该抗体在三阴性乳腺癌组织中检测到磷酸化水平与化疗耐药性正相关。

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**提示**：以上为模拟文献，实际文献检索建议通过PubMed或Web of Science以“Caspase-3 Phospho-Ser150 antibody”为关键词查询，重点关注《Cell Death & Differentiation》《Journal of Biological Chemistry》等期刊的研究。

背景信息

Caspase-3. a key executioner protease in apoptosis, is activated through cleavage of its inactive zymogen (procaspase-3) during programmed cell death. Phosphorylation at specific residues, such as Serine 150 (Ser150), is a critical regulatory mechanism modulating its activity. The Caspase-3 (Phospho-Ser150) antibody specifically detects caspase-3 when phosphorylated at this site, enabling researchers to study post-translational modifications that influence its function.

Phosphorylation of Ser150 in caspase-3 has been linked to inhibitory effects on its proteolytic activation. Studies suggest this modification may delay or suppress apoptosis under certain conditions, such as DNA damage, by interfering with caspase-3 processing. This regulatory mechanism is thought to involve kinases like ATM/ATR, which phosphorylate Ser150 in response to cellular stress, providing a survival signal to allow DNA repair before committing to apoptosis.

The Caspase-3 (Phospho-Ser150) antibody is widely used in immunoblotting, immunofluorescence, and immunohistochemistry to investigate apoptotic regulation in cancer, neurodegenerative diseases, and developmental biology. It helps differentiate between inactive phosphorylated caspase-3 and its activated (cleaved) form, offering insights into cellular stress responses and therapeutic targeting. Its specificity makes it a valuable tool for exploring phosphorylation-dependent apoptosis pathways and biomarker discovery.