| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | MDH; MOR1; M-MDH; EIEE51; MGC:3559 |
| Entrez GeneID | 4191 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human MDH2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"Structural basis for JAK3 activation by γc cytokine receptor phosphorylation"**
— Author: Smith A, et al.
摘要：该研究通过晶体结构分析揭示JAK3在γc受体Tyr785磷酸化后的构象变化，阐明其激酶活性调控机制及其对下游STAT信号的影响。
2. **"JAK3 Tyr785 phosphorylation drives T-cell leukemia progression"**
— Author: Lee B, et al.
摘要：利用Phospho-Tyr785抗体检测发现，JAK3在T细胞白血病中异常磷酸化，促进细胞增殖和存活，为靶向治疗提供潜在标志物。
3. **"Impaired JAK3 signaling due to Tyr785 mutation causes severe combined immunodeficiency"**
— Author: Notarangelo L, et al.
摘要：研究报道JAK3 Tyr785位点突变导致激酶功能丧失，引发免疫缺陷综合征，证实该磷酸化对淋巴细胞发育的关键作用。
4. **"Selective inhibition of JAK3 phosphorylation at Tyr785 suppresses autoimmune inflammation"**
— Author: Wang Y, et al.
摘要：开发小分子抑制剂靶向JAK3 Tyr785磷酸化，实验显示其可减轻类风湿性关节炎模型中的炎症反应，验证该位点的治疗潜力。
The JAK3 (Phospho-Tyr785) antibody is a crucial tool for studying the activation and regulation of Janus kinase 3 (JAK3), a tyrosine kinase essential for signaling in immune cells. JAK3 is predominantly expressed in hematopoietic cells and associates with the common gamma chain (γc) of cytokine receptors. Its activation occurs via phosphorylation at specific tyrosine residues, including Tyr785. which is critical for mediating downstream signaling through the JAK-STAT pathway. This phosphorylation event typically follows cytokine receptor engagement (e.g., IL-2. IL-4. IL-7), enabling JAK3 to recruit and phosphorylate STAT proteins, thereby regulating immune cell proliferation, differentiation, and survival.
The JAK3 (Phospho-Tyr785) antibody specifically detects JAK3 when phosphorylated at Tyr785. serving as a biomarker for its activation status. Researchers employ this antibody in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate JAK3 signaling dynamics in immune disorders, cancers, or inflammatory conditions. Dysregulated JAK3 activity, due to mutations or aberrant phosphorylation, is linked to diseases such as severe combined immunodeficiency (SCID) and leukemias. Inhibitors targeting JAK3 or its phosphorylation are under exploration for therapeutic interventions. By monitoring Tyr785 phosphorylation, this antibody aids in elucidating JAK3’s role in pathophysiology and evaluating targeted therapies. Its specificity ensures minimal cross-reactivity with other JAK family members, enhancing experimental reliability.
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