| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas |
| Entrez GeneID | 6198; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of S418. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于p70S6 kinase α (Phospho-Ser418)抗体的3篇参考文献示例(注：具体文献可能需根据实际研究调整，以下为模拟内容)：
1. **文献名称**: "Regulation of p70 S6 kinase by phosphorylation in response to mitogenic signals"
**作者**: Dennis, P.B. et al.
**摘要**: 研究揭示了p70S6 kinase α在Ser418等位点的磷酸化对酶活性的调控作用，证明该位点的磷酸化是响应生长因子激活mTOR通路的关键步骤，并促进核糖体生物合成和细胞增殖。
2. **文献名称**: "Identification of phosphorylation sites in the translational regulator, p70 S6 kinase"
**作者**: Pullen, N. & Thomas, G.
**摘要**: 通过质谱分析和定点突变技术，确认了p70S6 kinase α的多个磷酸化位点，包括Ser418.研究指出，Ser418磷酸化抗体可用于检测胰岛素或血清刺激下该激酶的活化状态，并与细胞周期进展相关。
3. **文献名称**: "The principal target of rapamycin-induced phosphorylation is the Ser/Thr kinase p70S6Kα"
**作者**: Pearson, R.B. et al.
**摘要**: 探讨了雷帕霉素对p70S6Kα的抑制作用，发现Ser418等位点的磷酸化水平与激酶活性直接相关。研究利用特异性磷酸化抗体证实，该位点的修饰是mTORC1信号调控翻译起始的关键标志。
(注：以上文献信息为示例，实际引用请核对真实数据库如PubMed、Google Scholar等。)
The p70S6 kinase α (Phospho-Ser418) antibody is designed to detect the phosphorylated form of p70S6 kinase α (also known as S6K1) at serine residue 418. a key regulatory site within its autoinhibitory domain. p70S6 kinase α is a serine/threonine kinase downstream of the mTORC1 (mechanistic target of rapamycin complex 1) signaling pathway, which integrates nutrient and growth factor signals to regulate cell growth, proliferation, and metabolism. Phosphorylation at Ser418. along with other residues (e.g., Thr389), relieves autoinhibition and activates the kinase, enabling it to phosphorylate ribosomal protein S6 and other substrates involved in mRNA translation and ribosome biogenesis.
This phosphorylation event is critical for cellular responses to insulin, amino acids, and mitogens, linking mTORC1 signaling to anabolic processes. Dysregulation of p70S6 kinase α activity is implicated in metabolic disorders, cancer, and aging. The Phospho-Ser418 antibody is widely used in research to assess mTORC1 pathway activation, study mechanisms of rapamycin (mTOR inhibitor) sensitivity, and investigate diseases associated with aberrant mTOR signaling. Specificity is validated through knockout controls or phosphatase treatment to confirm phosphorylation-dependent detection. Applications include Western blotting, immunohistochemistry, and immunofluorescence in cell/tissue samples.
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