Rabbit Polyclonal EF-2(Phospho-Thr56) Antibody

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     Western Blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody    

The EF-2 (Phospho-Thr56) antibody is designed to detect elongation factor 2 (EF-2) when phosphorylated at threonine 56. a post-translational modification critical for regulating protein synthesis. EF-2 is a GTP-binding protein essential for the translocation step during mRNA translation, facilitating the movement of tRNA and mRNA within the ribosome. Phosphorylation at Thr56 is catalyzed by EF-2 kinase (eEF2K), which inhibits EF-2’s activity, thereby temporarily halting elongation and reducing global protein synthesis. This regulatory mechanism is often activated under stress conditions, such as nutrient deprivation, hypoxia, or energy depletion, to conserve cellular resources.

The antibody serves as a key tool for studying translational control, cellular stress responses, and signaling pathways involving eEF2K. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess EF-2 phosphorylation status in various experimental models. Dysregulation of EF-2 phosphorylation has been implicated in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, where aberrant protein synthesis or stress adaptation pathways contribute to pathogenesis. Researchers also utilize this antibody to investigate therapeutic interventions targeting translation machinery or eEF2K activity. Specificity for the phosphorylated Thr56 epitope ensures minimal cross-reactivity, making it reliable for distinguishing active/inactive EF-2 states in complex biological samples.