| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA |
| WB Predicted band size | 83 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HADHA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Topo IIα (Phospho-Ser1525)抗体的3篇参考文献示例(注：文献为模拟示例，具体内容需根据实际研究补充)：
1. **文献名称**：*Phosphorylation of Topoisomerase IIα at Serine 1525 Modulates DNA Decatenation and Mitotic Progression*
**作者**：Nitiss, J. L. et al.
**摘要**：研究揭示了Ser1525磷酸化在DNA解连环和有丝分裂中的调控作用，使用Phospho-Ser1525抗体证实该位点磷酸化水平与染色体分离缺陷相关。
2. **文献名称**：*DNA Damage-Induced Phosphorylation of Topo IIα at Ser1525 Impairs Enzyme Activity*
**作者**：Ishikawa, K. et al.
**摘要**：通过Phospho-Ser1525特异性抗体，发现DNA损伤后该位点磷酸化抑制Topo IIα的催化活性，影响细胞修复能力。
3. **文献名称**：*A Phospho-Specific Antibody for Topo IIα Ser1525 Reveals Its Role in Chemotherapy Resistance*
**作者**：Kobayashi, T. & Andoh, T.
**摘要**：利用该抗体证明肿瘤细胞中Ser1525异常磷酸化与依托泊苷耐药性相关，为临床治疗提供潜在标志物。
如需真实文献，建议在PubMed或Web of Science中搜索关键词“Topo IIα Ser1525 phosphorylation antibody”或联系抗体供应商获取引用文献列表。
Topoisomerase IIα (Topo IIα) is a critical enzyme involved in DNA replication, transcription, and chromosome segregation by resolving topological stress in double-stranded DNA. Its activity is tightly regulated during the cell cycle, peaking in the G2/M phase to ensure proper chromosome condensation and sister chromatid separation. Post-translational modifications, including phosphorylation, play a key role in modulating Topo IIα function. Phosphorylation at Ser1525. located in the C-terminal domain, is associated with enzyme activation and chromatin binding during mitosis. This modification is thought to facilitate interactions with regulatory proteins or DNA, ensuring timely decatenation of replicated chromosomes.
The Topo IIα (Phospho-Ser1525) antibody specifically detects Topo IIα when phosphorylated at Ser1525. serving as a tool to study cell cycle dynamics, particularly mitotic progression. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or flow cytometry to monitor phosphorylation status in synchronized cells or during checkpoint activation. Aberrant Topo IIα activity or regulation is linked to genomic instability and diseases, including cancer. Overexpression of Topo IIα in rapidly dividing tumor cells makes it a target for chemotherapeutic agents like etoposide. Studying Ser1525 phosphorylation may provide insights into drug resistance mechanisms or mitotic errors in malignancies. Validation of this antibody typically includes testing in cell models with induced mitosis or phosphatase-treated lysates to confirm specificity for the phosphorylated epitope.
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