| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | MAP2K7; JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; S |
| Entrez GeneID | 5609; |
| WB Predicted band size | 47kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human MEK-7 around the phosphorylation site of S271. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MEK-7 (Phospho-Ser271)抗体的3篇参考文献的简要概括(注：MEK-7可能为MKK7/MAP2K7的别称，需结合文献验证具体磷酸化位点)：
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1. **文献名称**：*Regulation of JNK signaling by the MKK7 phosphorylation switch*
**作者**：Zheng-Feng Xu, et al.
**摘要**：研究揭示了MKK7在Ser271位点的磷酸化对其激酶活性的调控作用，通过特异性抗体(Phospho-Ser271)检测发现，该位点的磷酸化可增强MKK7对JNK通路的激活能力，并促进细胞凋亡。
2. **文献名称**：*Phosphorylation-dependent scaffolding role of MKK7 in TLR4 signaling*
**作者**：Jia Li, et al.
**摘要**：利用Phospho-Ser271抗体验证了MKK7在炎症反应中的功能，发现TLR4激活后，MKK7的Ser271磷酸化促进其与TAB1的相互作用，从而调控NF-κB和JNK信号通路的协同激活。
3. **文献名称**：*Antibody-based detection of MKK7 phosphorylation in tumor samples*
**作者**：Satoshi Hirano, et al.
**摘要**：开发了一种针对MKK7 Ser271磷酸化位点的单克隆抗体，通过免疫组化证实其在多种癌症组织中的高表达，提示该位点可能作为肿瘤预后的潜在标志物。
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**提示**：以上文献为模拟概括，实际引用需通过数据库(如PubMed)以关键词“MKK7 Ser271 phosphorylation”或“MAP2K7 p-Ser271”检索并核实。部分公司抗体说明书(如CST #4171)可能提供相关参考文献。
The MEK-7 (Phospho-Ser271) antibody is designed to detect the phosphorylated form of MAP kinase kinase 7 (MKK7) at serine residue 271. MKK7. a member of the mitogen-activated protein kinase kinase (MAP2K) family, is a critical upstream activator of c-Jun N-terminal kinase (JNK) signaling pathways. Phosphorylation at Ser271. located within the kinase domain’s activation loop, is essential for MKK7’s enzymatic activity. This post-translational modification typically occurs in response to cellular stressors (e.g., cytokines, UV radiation, or oxidative stress), enabling MKK7 to phosphorylate and activate JNKs, which subsequently regulate apoptosis, inflammation, and differentiation processes.
The MEK-7 (Phospho-Ser271) antibody is widely used in immunoblotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to study JNK pathway activation in diseases like cancer, neurodegenerative disorders, and immune dysregulation. Researchers employ this antibody to investigate context-specific MKK7 regulatory mechanisms, as aberrant JNK signaling is linked to tumor progression, neuronal death, and chronic inflammation. Specificity validation via knockout/knockdown controls is recommended to confirm target recognition. Commercial suppliers often provide datasheets detailing applications, species reactivity, and storage conditions.
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