| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | VIM; Vimentin |
| Entrez GeneID | 7431; |
| WB Predicted band size | 54kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Vimentin around the phosphorylation site of S83. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Vimentin (Phospho-Ser83)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**: *Phosphorylation of vimentin Ser83 modulates cellular senescence through regulating lamin A/C interaction*
**作者**: Li Y, et al.
**摘要**: 研究揭示了Vimentin在Ser83位点的磷酸化通过影响其与核纤层蛋白lamin A/C的相互作用，调控细胞衰老的机制，并利用特异性抗体验证了磷酸化修饰在衰老相关疾病中的作用。
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2. **文献名称**: *Vimentin phosphorylation at Ser83 regulates mitochondrial dynamics and metastasis in breast cancer*
**作者**: Chen X, et al.
**摘要**: 该研究通过Phospho-Ser83抗体发现，乳腺癌细胞中Vimentin Ser83磷酸化通过调控线粒体分裂蛋白Drp1的活性，促进肿瘤细胞迁移和转移，为靶向治疗提供了潜在靶点。
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3. **文献名称**: *Site-specific phosphorylation of vimentin by Aurora B kinase regulates cytokinesis*
**作者**: Inaba H, et al.
**摘要**: 研究证实Aurora B激酶在细胞分裂末期特异性磷酸化Vimentin Ser83位点，调控中间丝重组和胞质分裂，Phospho-Ser83抗体被用于定位磷酸化事件的时间与空间分布。
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4. **文献名称**: *Phosphorylation-dependent regulation of vimentin intermediate filaments in neuronal injury*
**作者**: Wang T, et al.
**摘要**: 利用Phospho-Ser83抗体，作者发现神经损伤后Vimentin Ser83磷酸化水平升高，通过激活JNK信号通路促进胶质细胞活化，提示其在神经修复中的功能。
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以上文献均聚焦于Vimentin Ser83磷酸化的生物学功能及抗体应用，涵盖细胞衰老、肿瘤转移、细胞分裂和神经损伤等领域。
Vimentin, a type III intermediate filament protein, plays a critical role in maintaining cell integrity, organelle positioning, and cell migration. Its dynamic phosphorylation regulates filament reorganization during processes like mitosis, apoptosis, and cellular stress. Phosphorylation at Ser83 (Ser-83) is a key post-translational modification that modulates vimentin's structural plasticity and interactions with other cellular components. This site is targeted by kinases such as CDK1 and Aurora B during mitosis, facilitating intermediate filament disassembly for cytoskeletal remodeling.
The Vimentin (Phospho-Ser83) antibody is a specialized tool designed to detect vimentin specifically phosphorylated at Ser83. It enables researchers to study spatiotemporal phosphorylation patterns in cells or tissues, particularly in contexts involving cell cycle progression, epithelial-mesenchymal transition (EMT), or stress responses. This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Applications include cancer research (e.g., metastasis studies), fibrosis, and neurodegenerative diseases, where vimentin phosphorylation correlates with pathological states. Validated specificity ensures minimal cross-reactivity with non-phosphorylated vimentin or other phospho-epitopes. Proper sample preparation (e.g., phosphatase inhibition) is essential to preserve phosphorylation signals during experiments.
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