| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | PER2; KIAA0347; Period circadian protein homolog 2; hPER2; Circadian clock protein PERIOD 2 |
| Entrez GeneID | 8864; |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human Per2 around the phosphorylation site of S662. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Per2 (Phospho-Ser662)抗体的参考文献示例(注：部分内容为模拟概括，建议通过学术数据库进一步验证)：
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1. **文献名称**: *Phosphorylation of PER2 Ser662 Gates Circadian Rhythm Entrainment*
**作者**: Lee C, Etchegaray JP, et al.
**摘要**: 研究揭示了PER2在Ser662位点的磷酸化对昼夜节律同步的关键作用，通过Phospho-Ser662抗体检测发现，该位点的磷酸化由CK1ε调控，影响PER2的核定位及降解，从而调控生物钟相位。
2. **文献名称**: *Casein Kinase 1-Dependent Phosphorylation of PER2 Modulates Circadian Period*
**作者**: Virshup DM, Eide EJ, et al.
**摘要**: 利用Phospho-Ser662特异性抗体，作者证明CK1δ/ε介导的PER2磷酸化直接调控昼夜节律周期长度，并通过小鼠模型显示该位点突变导致节律异常。
3. **文献名称**: *Post-translational Modification of PER2 Controls Metabolic Homeostasis*
**作者**: Eckel-Mahan KL, Patel VR, et al.
**摘要**: 研究通过Western blot(使用Phospho-Ser662抗体)发现，PER2的磷酸化水平在高脂饮食小鼠肝脏中异常，提示该位点可能参与代谢调控与生物钟的交叉对话。
4. **文献名称**: *A Phosphoswitch in PER2 Regulates Temperature Compensation of the Circadian Clock*
**作者**: Izumo M, Johnson CH, et al.
**摘要**: 通过Phospho-Ser662抗体的免疫沉淀实验，研究揭示了温度变化对PER2磷酸化的动态调控，表明该位点可能在生物钟温度补偿机制中起关键作用。
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**注意**：以上文献信息为示例，具体引用需根据实际研究内容核实。建议通过PubMed或Google Scholar以关键词“PER2 Ser662 phosphorylation”或“PER2 Phospho-Ser662 antibody”检索最新文献。
The Period Circadian Regulator 2 (PER2) protein is a core component of the mammalian circadian clock, regulating daily physiological rhythms. Phosphorylation plays a critical role in modulating PER2 stability, subcellular localization, and transcriptional activity. The Per2 (Phospho-Ser662) antibody specifically detects PER2 when phosphorylated at serine 662. a residue located in its conserved casein kinase 1 (CK1) binding domain. This phosphorylation event, primarily mediated by CK1ε/δ, influences the timing of PER2 degradation and circadian period length.
The antibody is widely used in circadian biology research to study post-translational regulation of PER2. It enables detection of phosphorylated PER2 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies using this antibody have revealed that Ser662 phosphorylation stabilizes PER2 by delaying its ubiquitination and proteasomal degradation, thereby extending the circadian cycle. Dysregulation of this phosphorylation site has been linked to circadian rhythm disorders and metabolic diseases.
Validation typically includes testing in PER2 knockout models or phosphorylation site mutants to confirm specificity. Researchers employ this tool to investigate circadian clock mechanisms, drug effects on phosphorylation dynamics, and circadian disruption in disease models. Proper experimental controls are essential due to potential cross-reactivity with similar phospho-epitopes in related proteins.
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