Rabbit Polyclonal TrkC(Phospho-Tyr516) Antibody

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     Western Blot analysis of HELA cells using Phospho-Trk C (Y516) Polyclonal Antibody    

The Trk C (Phospho-Tyr516) antibody is a specialized tool used to detect the activated form of Tropomyosin receptor kinase C (Trk C), a member of the Trk receptor tyrosine kinase family encoded by the *NTRK3* gene. Trk C plays a critical role in nervous system development, neuronal survival, and synaptic plasticity by binding to its primary ligand, neurotrophin-3 (NT-3). Upon ligand binding, Trk C undergoes dimerization and autophosphorylation at specific tyrosine residues, including Tyr516. within its intracellular kinase domain. Phosphorylation at Tyr516 is a key regulatory event that activates downstream signaling pathways such as MAPK, PI3K-Akt, and PLC-γ, which mediate cellular processes like differentiation, growth, and apoptosis.

This antibody specifically recognizes Trk C when phosphorylated at Tyr516. making it valuable for studying receptor activation dynamics in physiological and pathological contexts. Researchers employ it in techniques like Western blotting, immunohistochemistry, or immunofluorescence to investigate Trk C signaling in neural development, neurodegenerative disorders, or cancer. Aberrant Trk C activation, often due to *NTRK3* gene fusions (e.g., ETV6-NTRK3), is implicated in certain tumors, driving uncontrolled proliferation and survival. The antibody thus aids in diagnosing Trk fusion-positive cancers and evaluating therapeutic responses to Trk inhibitors. Its specificity for the phosphorylated state ensures precise detection of active Trk C, distinguishing it from the inactive form, which is crucial for mechanistic studies and drug development targeting Trk pathways.