| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 1/100-1/300 | Human,Mouse,Rat Monkey |
| ICC | 1/200-1/1000 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 1/10000 | Human,Mouse,Rat Monkey |
| Aliases | POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1 |
| Entrez GeneID | 5430; |
| WB Predicted band size | 250kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Synthesized peptide derived from human Rpb1 around the phosphorylation site of S1619. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于 Rpb1 (Phospho-Ser1619) 抗体的参考文献示例(文献标题和内容为虚构示例，仅用于展示格式)：
1. **"Phosphorylation of RNA Polymerase II CTD at Ser1619 Regulates Transcription Termination"**
*Author: Lee et al. (2018)*
摘要：本研究利用 Rpb1 (Phospho-Ser1619) 特异性抗体，揭示了 RNA 聚合酶 II C 端结构域(CTD)在 Ser1619 位点的磷酸化对转录终止的调控作用，并通过 ChIP-seq 分析证明其与终止因子相互作用。
2. **"DNA Damage-Induced Phosphorylation of Rpb1 at Ser1619 Promotes Genome Stability"**
*Author: Martinez et al. (2020)*
摘要：通过 Western blot 和免疫荧光实验，作者使用该抗体证明 DNA 损伤(如电离辐射)会诱导 Ser1619 磷酸化，并发现其与同源重组修复通路的关键蛋白存在共定位。
3. **"A Novel Role of Rpb1 Ser1619 Phosphorylation in mRNA Splicing"**
*Author: Chen & Wang (2019)*
摘要：研究通过抑制 Ser1619 磷酸化或使用特异性抗体阻断其功能，发现该位点的修饰影响 pre-mRNA 剪接效率，提示 CTD 磷酸化在转录-剪接偶联中的新机制。
4. **"Development and Validation of a Phospho-Specific Antibody for Rpb1 Ser1619"**
*Author: Johnson et al. (2016)*
摘要：本文详细描述了 Rpb1 (Phospho-Ser1619) 抗体的制备与验证，包括其在多种细胞模型中的特异性检测，并应用于研究细胞周期依赖的磷酸化动态变化。
(注：上述文献为模拟内容，实际文献需通过 PubMed 或 Google Scholar 检索关键词 "Rpb1 Ser1619 phosphorylation" 或 "RNA polymerase II CTD phospho-Ser1619" 获取。)
The Rpb1 (Phospho-Ser1619) antibody is a specialized tool used to detect the phosphorylation of serine 1619 on the largest subunit of RNA polymerase II (RNAP II), Rpb1 (also known as POLR2A). RNAP II is essential for transcribing protein-coding genes, and its activity is tightly regulated by post-translational modifications, particularly phosphorylation of its C-terminal domain (CTD). The CTD consists of heptapeptide repeats that undergo dynamic phosphorylation during transcription initiation, elongation, and termination. Phosphorylation at Ser1619. located near the CTD, has been implicated in transcription-coupled DNA damage repair and stress response pathways. This modification is thought to play a role in signaling DNA lesions, recruiting repair factors, or modulating transcriptional pausing.
The Rpb1 (Phospho-Ser1619) antibody specifically recognizes this phosphorylation event, enabling researchers to study its regulatory role in transcription under stress conditions, such as UV irradiation or genotoxic agent exposure. It is widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate transcriptional dynamics, DNA repair mechanisms, and cellular responses to genomic instability. Understanding Ser1619 phosphorylation provides insights into diseases linked to transcription or DNA repair dysregulation, including cancer and neurodegenerative disorders. This antibody serves as a critical reagent for dissecting RNAP II's functional versatility in gene expression and genome maintenance.
×
