| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 1/100-1/300 | Human,Mouse,Rat Monkey |
| ICC | 1/200-1/1000 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 1/5000 | Human,Mouse,Rat Monkey |
| Aliases | CRKL; Crk-like protein |
| Entrez GeneID | 1399; |
| WB Predicted band size | 39kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Synthesized peptide derived from human Crk-L around the phosphorylation site of Y207. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Crk-L (Phospho-Tyr207)抗体的参考文献示例(文献信息为模拟示例，仅供参考)：
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1. **文献名称**：*Phosphorylation of CrkL at Tyr207 regulates its interaction with C3G in EGF signaling*
**作者**：Matsuda K, et al.
**摘要**：研究揭示了EGF刺激下Crk-L在Tyr207位点的磷酸化如何调控其与C3G的相互作用，从而影响细胞迁移。该文献使用Phospho-Tyr207特异性抗体验证了磷酸化事件及其功能关联。
2. **文献名称**：*CrkL tyrosine phosphorylation in cancer progression: Implications for metastatic invasion*
**作者**：Ota T, et al.
**摘要**：通过Phospho-Tyr207抗体检测，发现Crk-L在多种癌细胞系中的异常磷酸化与肿瘤侵袭性增强相关，表明其作为潜在治疗靶点的价值。
3. **文献名称**：*Role of CrkL phosphorylation in T-cell receptor signaling*
**作者**：Feller SM, et al.
**摘要**：利用Tyr207磷酸化特异性抗体，证实T细胞激活过程中Crk-L的磷酸化通过调节DOCK180复合物形成，影响免疫信号通路的活化。
4. **文献名称**：*Targeting CrkL phosphorylation for therapeutic intervention in fibrosis*
**作者**：Wu Y, et al.
**摘要**：研究开发了一种抑制Crk-L Tyr207磷酸化的小分子化合物，并通过抗体检测验证其有效性，为纤维化疾病提供了新策略。
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注：以上文献为示例性内容，实际引用时请根据真实发表的论文核实信息。建议通过PubMed或Google Scholar以关键词“Crk-L Tyr207 phosphorylation”或“Crk-L pY207 antibody”检索最新文献。
The Crk-L (Phospho-Tyr207) antibody is a specialized tool used to detect the phosphorylation status of the Crk-like (Crk-L) protein at tyrosine residue 207. Crk-L, a member of the Crk adaptor protein family, plays a critical role in intracellular signaling by mediating protein-protein interactions through its SH2 and SH3 domains. These interactions facilitate signal transduction pathways involved in cell adhesion, migration, proliferation, and survival, often linking tyrosine kinases to downstream effectors like Ras and Rac. Phosphorylation at Tyr207 is a key regulatory mechanism that modulates Crk-L’s activity, influencing its binding affinity for partner proteins and its subcellular localization.
This phosphorylation event is typically induced by upstream kinases (e.g., Abl, EGFR) in response to extracellular stimuli such as growth factors or cellular stress. Dysregulation of Crk-L phosphorylation has been implicated in pathological conditions, including cancer and inflammatory diseases, where aberrant signaling promotes tumor invasiveness or immune dysfunction. The Crk-L (Phospho-Tyr207) antibody enables researchers to assess the activation state of Crk-L in experimental models, providing insights into signaling dynamics in normal physiology or disease contexts. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specificity validation and proper controls are essential to ensure accurate detection, given the structural homology between Crk-L and related family members like Crk-II.
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