| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1 |
| Entrez GeneID | 2549; |
| WB Predicted band size | 76kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Gab 1 around the phosphorylation site of Y659. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Gab1 (Phospho-Tyr659)抗体的3篇示例参考文献(注：以下为示例性内容，实际文献需根据具体研究补充)：
1. **"Role of Gab1 phosphorylation in Met-mediated signaling pathways"**
- **作者**: Sachs M. et al.
- **摘要**: 研究Gab1酪氨酸磷酸化(包括Tyr659位点)在Met受体激活后的信号传导作用，证实该位点磷酸化对PI3K/Akt通路激活及细胞迁移至关重要。
2. **"EGF-induced Gab1 tyrosine phosphorylation recruits downstream effectors"**
- **作者**: Rodrigues G.A. et al.
- **摘要**: 通过免疫沉淀结合Phospho-Tyr659抗体，揭示EGF刺激下Gab1在Tyr659位点的磷酸化促进Grb2和SHP2结合，调控MAPK通路活性。
3. **"Gab1 phosphorylation status in cancer cell proliferation"**
- **作者**: Fan S. et al.
- **摘要**: 使用特异性Phospho-Tyr659抗体分析乳腺癌细胞中Gab1的异常磷酸化，发现其与HER2过表达相关，并影响肿瘤细胞的侵袭能力。
建议通过PubMed或Google Scholar以关键词“Gab1 Tyr659 phosphorylation”或“Gab1 phospho-specific antibody”查询最新研究。实际研究中需结合抗体品牌(如CST、Abcam)提供的引用文献。
The Gab1 (Phospho-Tyr659) antibody is designed to detect endogenous levels of Gab1 protein when phosphorylated at tyrosine residue 659. Gab1 (GRB2-associated binding protein 1) is a scaffolding adaptor protein involved in multiple signaling pathways, including those activated by receptor tyrosine kinases (RTKs), cytokine receptors, and integrins. It contains a pleckstrin homology (PH) domain, which mediates membrane localization, and multiple binding sites for signaling molecules such as PI3K, SHP2. and CRKL. Phosphorylation of Tyr659 in Gab1 is critical for its role in signal transduction. This phosphorylation event typically occurs in response to growth factor or cytokine stimulation (e.g., EGF, HGF, or insulin), facilitating the recruitment of downstream effectors containing SH2 domains, such as Grb2. which further propagate signals to pathways like MAPK/ERK and PI3K/Akt.
The Gab1 (Phospho-Tyr659) antibody is widely used in research to study Gab1 activation dynamics in cellular processes like proliferation, differentiation, and survival. It is commonly applied in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess phosphorylation status in cell lysates or tissues. Dysregulation of Gab1 signaling has been implicated in cancer, inflammation, and metabolic disorders, making this antibody a valuable tool for investigating disease mechanisms and therapeutic targets. Specificity is typically validated using phosphorylation-deficient mutants or phosphopeptide competition assays.
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