Rabbit Polyclonal DREAM(Phospho-Ser63) Antibody

中文名： DREAM (Phospho-Ser63)抗体
别名： KCNIP3; CSEN; DREAM; KCHIP3; Calsenilin; A-type potassium channel modulatory protein 3; DRE-antagonist modulator; DREAM; Kv channel-interacting protein 3; KChIP3

货号: IPDX40961

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/300	Human,Mouse,Rat
ICC	1/200-1/1000	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	1/20000	Human,Mouse,Rat

产品详情

Aliases	KCNIP3; CSEN; DREAM; KCHIP3; Calsenilin; A-type potassium channel modulatory protein 3; DRE-antagonist modulator; DREAM; Kv channel-interacting protein 3; KChIP3
Entrez GeneID	30818;
WB Predicted band size	29kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthesized peptide derived from human DREAM around the phosphorylation site of S63.
Formulation	Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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Western blot analysis of SH-SY5Y 293T 3T3 lysis using Phospho-DREAM (S63) antibody.

参考文献

以下是关于DREAM (Phospho-Ser63)抗体的3篇虚构参考文献示例，内容参考了相关领域常见研究主题：

1. **文献名称**：Phosphorylation of DREAM at Ser63 Modulates Pain Sensitivity in a Murine Neuropathic Pain Model

**作者**：Chen L., et al.

**摘要**：该研究通过Western blot和免疫组化使用Phospho-Ser63 DREAM抗体，发现神经损伤模型中DREAM蛋白在脊髓背角神经元的Ser63磷酸化水平显著升高。磷酸化导致DREAM与DNA解离，促进下游痛觉相关基因(如prodynorphin)表达，提示该位点磷酸化可能是疼痛调控的关键靶点。

2. **文献名称**：DREAM Phosphorylation at Ser63 Mediates Neuroprotection in Ischemic Stroke

**作者**：Park S., et al.

**摘要**：研究者利用Phospho-Ser63特异性抗体发现，小鼠脑缺血后皮层神经元中DREAM的Ser63磷酸化水平快速上升，导致其从靶基因启动子区域解离，激活抗凋亡基因(如BDNF)。敲除磷酸化位点显著加重神经元损伤，证明该修饰在缺血预适应中起保护作用。

3. **文献名称**：Calcium-Dependent Kinase Regulation of DREAM via Ser63 Phosphorylation in Cardiac Hypertrophy

**作者**：Gomez M., et al.

**摘要**：通过免疫沉淀结合Phospho-Ser63抗体，研究发现钙调蛋白激酶II(CaMKII)在心肌细胞肥大模型中催化DREAM的Ser63磷酸化，抑制其与Ncx1基因启动子结合，从而上调钠钙交换蛋白表达。此通路可能成为心力衰竭治疗的干预靶点。

注：以上文献为示例性虚构内容，实际研究中建议通过PubMed或Google Scholar检索真实文献。

背景信息

The DREAM (Downstream Regulatory Element Antagonist Modulator) protein, also known as calsenilin or KChIP3. is a calcium-binding protein belonging to the neuronal calcium sensor family. It functions as a transcriptional repressor by binding to downstream regulatory elements (DREs) in target gene promoters, including genes involved in pain modulation, neuronal plasticity, and apoptosis. Phosphorylation at Serine 63 (Ser63) is a critical post-translational modification regulating DREAM activity. This phosphorylation, mediated by kinases such as protein kinase A (PKA) or protein kinase C (PKC), disrupts DREAM's DNA-binding capacity, thereby relieving transcriptional repression and enabling gene expression.

The DREAM (Phospho-Ser63) antibody is a specialized tool designed to detect endogenous DREAM protein phosphorylated specifically at Ser63. It is widely used in neuroscience and molecular biology research to study calcium-dependent signaling pathways, gene regulation mechanisms, and cellular responses to stimuli like oxidative stress or neuronal injury. This antibody aids in elucidating DREAM's role in physiological processes (e.g., pain perception, memory formation) and pathological conditions, including neurodegenerative diseases, chronic pain syndromes, and cancer. Its specificity for the phosphorylated form allows researchers to distinguish activated DREAM from its unmodified state, providing insights into dynamic regulatory mechanisms. Validation typically includes Western blotting, immunohistochemistry, and immunofluorescence, ensuring reliability across experimental models.