| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | FANCA; FAA; FACA; FANCH; Fanconi anemia group A protein; Protein FACA |
| Entrez GeneID | 2175; |
| WB Predicted band size | 162kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human FANCA around the phosphorylation site of S1149. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于FANCA (Phospho-Ser1149)抗体的3篇参考文献的示例(注：文献为模拟示例，实际引用需核实数据库)：
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1. **文献名称**: *"Phosphorylation of FANCA at Serine 1149 is essential for DNA damage-induced foci formation"*
**作者**: Smith J, et al.
**摘要**: 本研究利用特异性识别FANCA磷酸化Ser1149位点的抗体，通过免疫荧光和Western blot分析，证实该位点的磷酸化是FANCA在DNA损伤后招募至染色质并形成核灶的关键步骤，提示其在Fanconi贫血通路中的调控作用。
2. **文献名称**: *"ATM-dependent phosphorylation of FANCA regulates its interaction with BRCA1"*
**作者**: Lee C, et al.
**摘要**: 作者使用Phospho-Ser1149特异性抗体，证明ATM激酶介导的FANCA磷酸化增强了其与BRCA1的结合能力，从而促进同源重组修复。研究通过免疫共沉淀和磷酸化缺失突变体验证了功能关联。
3. **文献名称**: *"A novel phospho-specific antibody reveals cell cycle-dependent phosphorylation of FANCA"*
**作者**: Tanaka R, et al.
**摘要**: 开发了一种高特异性抗FANCA p-Ser1149的多克隆抗体，并发现该位点的磷酸化水平在S/G2期显著升高，提示其与复制应激响应相关。研究结合流式细胞术和磷酸化抗体验证了这一现象。
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**备注**：
以上文献为示例性质，实际研究中建议通过 **PubMed/Google Scholar** 以关键词 `FANCA Phospho-Ser1149 antibody` 或 `FANCA Ser1149 phosphorylation` 检索最新文献，并优先选择抗体验证数据完整(如KO细胞验证、肽竞争实验)的研究。部分公司(如CST、Abcam)的抗体说明书也可能提供引用文献。
The FANCA (Phospho-Ser1149) antibody detects the phosphorylated form of FANCA (Fanconi anemia complementation group A) protein at serine residue 1149. FANCA is a critical component of the Fanconi anemia (FA) core complex, which plays a central role in DNA interstrand crosslink repair and maintaining genomic stability. Phosphorylation at Ser1149 is associated with the activation or regulatory mechanisms of the FA pathway, particularly in response to DNA damage. The FA pathway coordinates with other DNA repair processes, such as homologous recombination, and its dysfunction leads to Fanconi anemia, a rare genetic disorder characterized by bone marrow failure, cancer predisposition, and developmental abnormalities.
This antibody is widely used in research to study FANCA’s post-translational modifications, its interaction with other FA complex proteins (e.g., FANCG, FANCC), and the molecular mechanisms underlying FA pathway activation. It helps investigate how phosphorylation events modulate FANCA’s function in DNA damage response, replication stress, or cell cycle regulation. Researchers employ it in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess FANCA activation status in cellular models, patient-derived samples, or cancer studies, where FA pathway defects are linked to chemoresistance or tumorigenesis. Its specificity for the phosphorylated epitope makes it valuable for dissecting signaling dynamics in Fanconi anemia and related pathologies.
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