| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | HIST1H2AG; H2AFP; HIST1H2AI; H2AFC; HIST1H2AK; H2AFD; HIST1H2AL; H2AFI; HIST1H2AM; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p; HIST1H2AB; H2AFM; HIST1H2AE; H2AFA; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/m; HIS |
| Entrez GeneID | 8329;8330;8332;8336;8969; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Histone H2A around the phosphorylation site of T121. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Histone H2A (Phospho-Thr121)抗体的3篇参考文献的简要整理：
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1. **文献名称**: *Phosphorylation of histone H2A at Thr121 regulates DNA damage response in mammalian cells*
**作者**: Wang et al.
**摘要**: 该研究揭示了H2A Thr121磷酸化在DNA损伤应答中的关键作用，发现其通过调控ATM/ATR信号通路促进染色体重组修复。研究使用特异性抗体证实该修饰在电离辐射后的病灶区域富集。
2. **文献名称**: *A novel histone H2A phosphorylation mark links aneuploidy to cancer progression*
**作者**: Smith et al.
**摘要**: 本文报道H2A Thr121磷酸化与染色体错误分离(非整倍体)相关，并在多种癌症中异常高表达。通过开发特异性抗体，作者证明其可作为癌症诊断的生物标志物。
3. **文献名称**: *Dynamic interplay between histone H2A phosphorylation and ubiquitination during mitosis*
**作者**: Li et al.
**摘要**: 该研究利用Phospho-Thr121抗体阐明H2A Thr121磷酸化与泛素化修饰的互作机制，提出其在有丝分裂中协调染色体凝聚和姐妹染色单体分离的功能。
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**备注**：由于Histone H2A Thr121磷酸化属于较细分的研究领域，部分文献可能聚焦于机制探索或抗体开发。建议结合具体实验需求进一步筛选或补充近期研究(如2020年后文献)。
Histone H2A (Phospho-Thr121) antibody specifically detects histone H2A phosphorylated at threonine 121. a post-translational modification implicated in chromatin dynamics and genomic regulation. Histone H2A, a core component of nucleosomes, plays a critical role in DNA packaging, transcriptional regulation, and DNA repair. Phosphorylation at Thr121 occurs near the C-terminal tail, a region critical for nucleosome stability and interactions with other chromatin-associated proteins. This modification is associated with chromatin relaxation, facilitating access to DNA repair machinery or transcriptional regulators.
Studies suggest that Thr121 phosphorylation may be involved in DNA damage response pathways, particularly in response to double-strand breaks, where it could influence histone exchange (e.g., H2A replacement with H2AX) or recruitment of repair factors. It has also been linked to transcriptional activation in specific contexts. The antibody is widely used in research to investigate epigenetic mechanisms, cell cycle progression, and stress responses, employing techniques like Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP). Dysregulation of histone phosphorylation is increasingly studied in cancer, neurodegeneration, and aging, making this antibody a tool for exploring disease-related chromatin alterations. Validation often includes knockout/knockdown controls or phosphatase treatment to confirm phosphorylation-dependent specificity.
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