| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | NR4A1; GFRP1; HMR; NAK1; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3 |
| Entrez GeneID | 3164; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Nur77 around the phosphorylation site of S351. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Nur77 (Phospho-Ser351)抗体的3篇参考文献示例，涵盖其在不同生物学背景中的应用：
1. **"Phosphorylation of Nur77 at Ser351 promotes mitochondrial translocation and apoptosis in T cells"**
- **作者**: Lin et al.
- **摘要**: 研究揭示了Nur77在T细胞受体信号通路中Ser351位点的磷酸化如何触发其从细胞核向线粒体转位，诱导细胞色素C释放和凋亡，强调了该抗体在检测磷酸化依赖性亚细胞定位中的作用。
2. **"NR4A1 phosphorylation at Ser351 modulates transcriptional activity in cancer cell proliferation"**
- **作者**: Zhang et al.
- **摘要**: 通过Phospho-Ser351抗体验证，发现Ser351磷酸化抑制Nur77的转录活性，进而抑制癌细胞增殖，表明该抗体可用于研究磷酸化修饰与肿瘤进展的关联。
3. **"Ser351 phosphorylation regulates Nur77-mediated gluconeogenesis in diabetic models"**
- **作者**: Wang et al.
- **摘要**: 利用Phospho-Ser351特异性抗体，阐明肝脏中Nur77的Ser351磷酸化通过招募共抑制因子抑制糖异生基因表达，为代谢疾病治疗提供新靶点。
(注：以上文献为示例，实际引用需根据具体研究补充真实来源。)
The Nur77 (Nuclear Receptor Subfamily 4 Group A Member 1. NR4A1) protein is a transcription factor belonging to the steroid/thyroid hormone receptor superfamily. It plays critical roles in apoptosis, inflammation, metabolism, and cancer progression. Phosphorylation at Serine 351 (Ser351) is a key post-translational modification regulating Nur77’s subcellular localization and functional activity. This modification is mediated by kinases such as ERK or JNK in response to cellular stress, growth signals, or apoptotic stimuli. Phosphorylated Nur77 (p-Ser351) undergoes nucleocytoplasmic translocation, interacting with mitochondrial proteins like Bcl-2 to induce cytochrome c release and apoptosis. Conversely, in some contexts, this phosphorylation may promote cell survival by modulating anti-apoptotic pathways.
The Nur77 (Phospho-Ser351) antibody is a specialized tool used to detect and quantify Nur77 phosphorylated at Ser351. It enables researchers to study the activation status of Nur77 in response to extracellular signals, cellular stress, or pharmacological interventions. Applications include Western blotting, immunofluorescence, and immunohistochemistry to explore Nur77’s role in diseases such as cancer, autoimmune disorders, and metabolic syndromes. Validated for specificity, this antibody helps dissect the dual pro- and anti-apoptotic functions of Nur77. offering insights into its context-dependent mechanisms and therapeutic targeting. Its utility spans basic research and preclinical studies, particularly in understanding cellular stress responses and drug resistance.
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