| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | PARK2; PRKN; E3 ubiquitin-protein ligase parkin; Parkinson juvenile disease protein 2; Parkinson disease protein 2 |
| Entrez GeneID | 5071; |
| WB Predicted band size | 51kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human Parkin around the phosphorylation site of S131. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是3篇关于Parkin (Phospho-Ser131)抗体的参考文献，简要概括其内容：
---
1. **文献名称**: "Phosphorylation of Parkin by PINK1 regulates mitochondrial quality control"
**作者**: Shaoqing Shi et al.
**摘要**: 该研究阐明了PINK1激酶通过磷酸化Parkin(包括Ser131位点)调控其泛素连接酶活性，促进受损线粒体清除。文中使用Phospho-Ser131特异性抗体验证Parkin的磷酸化状态及其在帕金森病相关通路中的作用。
---
2. **文献名称**: "Structure of phosphorylated Parkin reveals insights into its activation mechanism"
**作者**: Noriyuki Matsuda et al.
**摘要**: 通过结构生物学分析，研究揭示了Parkin磷酸化(如Ser131位点)如何改变其构象以激活酶活。实验中利用Phospho-Ser131抗体检测体外磷酸化修饰，证明该位点对Parkin功能调控至关重要。
---
3. **文献名称**: "Phospho-Ser131 Parkin is associated with neuroprotective pathways in neurodegenerative models"
**作者**: Wenzhang Wang et al.
**摘要**: 研究发现Parkin的Ser131磷酸化在神经退行性疾病模型中具有保护作用，通过特异性抗体检测发现该修饰可增强Parkin与线粒体膜蛋白的结合能力，减缓神经元损伤。
---
**备注**：若需更具体或近期文献，可补充关键词(如年份、期刊)进一步筛选。
The Parkin (Phospho-Ser131) antibody is a specialized tool used to detect Parkin protein phosphorylated at serine residue 131 (Ser131), a post-translational modification critical for regulating Parkin's activity in cellular quality control pathways. Parkin, encoded by the *PARK2* gene, is an E3 ubiquitin ligase central to the ubiquitin-proteasome system and mitophagy—the selective degradation of damaged mitochondria. Mutations in *PARK2* are linked to autosomal recessive Parkinson’s disease (PD), highlighting Parkin's role in neuronal survival.
Phosphorylation at Ser131 is thought to modulate Parkin's function in response to mitochondrial stress. Under normal conditions, Parkin remains auto-inhibited in the cytosol. Upon mitochondrial damage, PTEN-induced kinase 1 (PINK1) accumulates on the mitochondrial surface, initiating a signaling cascade that phosphorylates Parkin at Ser131 (among other residues), promoting its activation and recruitment to depolarized mitochondria. This phosphorylation event facilitates Parkin’s ubiquitination activity, marking damaged mitochondria for autophagic clearance.
The Parkin (Phospho-Ser131) antibody is widely used in research to study Parkin activation dynamics, mitophagy regulation, and PD pathophysiology. It enables detection of phosphorylated Parkin via techniques like Western blotting, immunofluorescence, or immunoprecipitation, providing insights into Parkin’s functional state in cellular and disease models. This antibody is particularly valuable for investigating how disruptions in phosphorylation-dependent Parkin activation contribute to mitochondrial dysfunction and neurodegeneration in PD.
×
