| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein |
| Entrez GeneID | 84152; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human DARPP-32 around the phosphorylation site of T34. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于DARPP-32 (Phospho-Thr34)抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"DARPP-32: A regulator of the efficacy of dopaminergic neurotransmission"*
**作者**：Greengard, P., et al.
**摘要**：该研究阐明了DARPP-32在纹状体多巴胺信号中的核心作用，指出Thr34磷酸化通过抑制蛋白磷酸酶PP-1调控下游效应。文中提及使用Phospho-Thr34特异性抗体验证多巴胺受体激活后DARPP-32磷酸化水平的变化，并通过基因敲除小鼠模型确认抗体特异性。
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2. **文献名称**：*"Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signaling in neurons"*
**作者**：Bibb, J.A., et al.
**摘要**：本文发现Cdk5与DARPP-32的Thr34磷酸化存在交叉调控，利用Phospho-Thr34抗体证明多巴胺与谷氨酸信号通路的相互作用。抗体通过Western blot和免疫组织化学验证，显示在神经元活性增强时Thr34位点磷酸化显著上调。
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3. **文献名称**：*"Role of phosphorylation of DARPP-32 in the nucleus accumbens in the actions of drugs of abuse"*
**作者**：Svenningsson, P., et al.
**摘要**：研究探讨了成瘾药物(如可卡因)对DARPP-32磷酸化的影响，通过Phospho-Thr34抗体检测发现药物暴露后纹状体神经元Thr34磷酸化水平升高，并关联到行为学变化。抗体特异性经肽段竞争实验和磷酸酶处理验证。
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**备注**：上述文献为示例性概括，实际引用时建议通过PubMed或Google Scholar以关键词“DARPP-32 Phospho-Thr34 antibody”检索最新研究，并核实抗体验证细节(如物种反应性、应用场景等)。
The DARPP-32 (Dopamine and cAMP-regulated phosphoprotein, 32 kDa) protein is a critical signaling integrator in medium spiny neurons of the striatum, modulating dopaminergic and glutamatergic pathways. Phosphorylation at threonine 34 (Thr34) by protein kinase A (PKA) enhances DARPP-32’s ability to inhibit protein phosphatase-1 (PP-1), amplifying dopamine receptor signaling. The DARPP-32 (Phospho-Thr34) antibody specifically detects this phosphorylation event, serving as a key tool to study dopamine-dependent neuroplasticity, reward mechanisms, and motor control.
This antibody is widely used in neuroscience research to investigate conditions involving dopaminergic dysregulation, such as addiction, Parkinson’s disease, schizophrenia, and depression. Phospho-Thr34 levels reflect PKA activity downstream of D1 dopamine receptors, making it a biomarker for dopamine signaling intensity. Applications include Western blotting, immunohistochemistry, and immunofluorescence in brain tissue or cell models.
Studies using this antibody have elucidated how psychostimulants (e.g., cocaine) or antipsychotics alter Thr34 phosphorylation, linking molecular changes to behavioral outcomes. Its specificity for the phosphorylated Thr34 epitope ensures accurate detection of activated DARPP-32. distinguishing it from other phosphorylation sites (e.g., Thr75). Validated in rodents, primates, and human samples, this antibody remains fundamental for exploring striatal signaling pathways and their roles in neuropsychiatric disorders.
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